|
|
| (16 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| {{STRUCTURE_1n3w| PDB=1n3w | SIZE=400| SCENE= |right|CAPTION=Maltose-binding protein complex with maltose, [[1n3w]] }}
| | <StructureSection load='1anf' size='350' side='right' caption='E. coli maltose-binding protein complex with maltose (PDB entry [[1anf]])' scene='47/471830/Cv/1'> |
| | == Function == |
| | '''Maltose-binding protein''' (MBP) or '''ABC maltose transporter''' is part of the maltose/maltodextrin system in ''E. coli'' which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport<ref>PMID:10452611</ref>. |
|
| |
|
| '''Maltose-binding protein''' (MBP) is part of the maltose/maltodextrin system in ''E. coli'' which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport. MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in ''E. coli'' by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose.
| | == Relevance == |
| | MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in ''E. coli'' by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose<ref>PMID:21125392</ref>. |
| | |
| | == Structural highlights == |
| | The <scene name='47/471830/Cv/5'>MBP sugar binding cleft is rich with protein-ligand hydrogen bonds</scene><ref>PMID:9309217</ref>. Water molecules are shown as red spheres. |
|
| |
|
| ==3D structures of maltose-binding protein== | | ==3D structures of maltose-binding protein== |
| | [[Maltose-binding protein 3D structures]] |
|
| |
|
| [[1llx]] - MBP<br />
| | </StructureSection> |
| [[3sey]], [[3sex]], [[3sew]], [[3sev]], [[3seu]], [[3set]], [[3ses]], [[3ser]], [[2v93]], [[1zkb]], [[1n3x]], [[1peb ]]– MBP (mutant)<br />
| |
| [[2klf]], [[2d21]] – MBP – NMR<br />
| |
| [[1ziu]] – MBP (mutant) + Ni<br />
| |
| [[1zjl]] – MBP (mutant) + Zn<br />
| |
| [[1zmg]] - MBP (mutant) + Cu
| |
| | |
| '''MBP complex with saccharide'''
| |
| | |
| [[1n3w]], [[1nl5]] - MBP (mutant) + maltose<br />
| |
| [[3hpi]] – MBP (mutant) + sucrose<br />
| |
| [[2h25]] - MBP + β-cyclodextrin<br />
| |
| [[1fqa]], [[1fqb]], [[1fqc]], [[1fqd]] - MBP + maltotetraitol
| |
| | |
| '''MBP complex with protein'''
| |
| | |
| [[3pgf]] – MBP (mutant) + synthetic antigen binder<br />
| |
| [[2r6g]] - MBP + maltose transporter complex<br />
| |
| [[3puv]], [[3puw]], [[3pux]] – MBP + maltose transporter complex + ADP <br />
| |
| [[3rlf]] - MBP + maltose transporter complex + Mg-AMPPNP<br />
| |
| [[1svx]] – MBP + ankyrin repeat protein OFF7
| |
| | |
| '''MBP fused to other proteins'''
| |
| | |
| [[3hst]] – MBP/RNase H domain<br />
| |
| [[3mq9]] – MBP/bone marrow stromal antigen 2<br />
| |
| [[3lbs]] - MBP/renin receptor + maltose<br />
| |
| [[3lc8]] - MBP/renin receptor<br />
| |
| [[3csb]] – MBP/monobody YSX1<br />
| |
| [[3csg]] - MBP/monobody YS1<br />
| |
| [[2obg]] - MBP/monobody MBP-74<br />
| |
| [[3f5f]] - MBP/Heparan sulfate 2-O-sulfotransferase<br />
| |
| [[3g7v]], [[3g7w]] - MBP/islet amyloid polypeptide<br />
| |
| [[2zxt]], [[3a3c]] - MBP/mitochondrial intermembrane space import and assembly protein 40<br />
| |
| [[1ytv]] - MBP/vasopressin receptor<br />
| |
| [[1r6z]]– MBP/argonaute 2<br />
| |
| [[1hsj]] - MBP/accessary regulator protein R<br />
| |
| [[3osq]], [[3osr]] – MBP/Green fluorescent protein
| |
|
| |
|
| | == References == |
| | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |