6fop: Difference between revisions

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-'''Unreleased structure'''
-The entry 6fop is ON HOLD
+==Glycoside hydrolase family 81 from Clostridium thermocellum (CtLam81A), Mutant E515A==
+<StructureSection load='6fop' size='340' side='right'caption='[[6fop]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6fop]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FOP FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cthe_0660 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 Clostridium thermocellum])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fop OCA], [http://pdbe.org/6fop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fop RCSB], [http://www.ebi.ac.uk/pdbsum/6fop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fop ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The family 81 glycoside hydrolase (GH81) from Clostridium thermocellum is a beta-1,3-glucanase belonging to cellulosomal complex. The gene encoding GH81 from Clostridium thermocellum (CtLam81A) was cloned and expressed displaying a molecular mass of ~82kDa. CtLam81A showed maximum activity against laminarin (100U/mg), followed by curdlan (65U/mg), at pH7.0 and 75 degrees C. CtLam81A displayed Km, 2.1+/-0.12mg/ml and Vmax, 109+/-1.8U/mg, against laminarin under optimized conditions. CtLam81A activity was significantly enhanced by Ca(2+) or Mg(2+) ions. Melting curve analysis of CtLam81A showed an increase in melting temperature from 91 degrees C to 96 degrees C by Ca(2+) or Mg(2+) ions and decreased to 82 degrees C by EDTA, indicating that Ca(2+) and Mg(2+) ions may be involved in catalysis and in maintaining structural integrity. TLC and MALDI-TOF analysis of beta-1,3-glucan hydrolysed products released initially, showed beta-1,3-glucan-oligosaccharides degree of polymerization (DP) from DP2 to DP7, confirming an endo-mode of action. The catalytically inactive mutant CtLam81A-E515A generated by site-directed mutagenesis was co-crystallized and tetragonal crystals diffracting up to 1.4A resolution were obtained. CtLam81A-E515A contained 15 alpha-helices and 38 beta-strands forming a four-domain structure viz. a beta-sandwich domain I at N-terminal, an alpha/beta-domain II, an (alpha/alpha)6 barrel domain III, and a small 5-stranded beta-sandwich domain IV.
-Authors: Correia, M.A.S.C., Carvalho, A.L.
+Novel insights into the degradation of beta-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolase.,Kumar K, Correia MAS, Pires VMR, Dhillon A, Sharma K, Rajulapati V, Fontes CMGA, Carvalho AL, Goyal A Int J Biol Macromol. 2018 Oct 1;117:890-901. doi: 10.1016/j.ijbiomac.2018.06.003., Epub 2018 Jun 2. PMID:29870811<ref>PMID:29870811</ref>
-Description: Glycoside hydrolase family 81 from Clostridium thermocellum (CtLam81A), Mutant E515A
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Correia, M.A.S.C]]
+<div class="pdbe-citations 6fop" style="background-color:#fffaf0;"></div>
-[[Category: Carvalho, A.L]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Clostridium thermocellum]]
+[[Category: Large Structures]]
+[[Category: Carvalho, A L]]
+[[Category: Correia, M A.S C]]
+[[Category: Gh81]]
+[[Category: Glucanase]]
+[[Category: Glycoside hydrolase]]
+[[Category: Hydrolase]]
+[[Category: Laminarin]]