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<StructureSection load='3cel' size='350' side='right' scene='48/488493/Cv/1' caption='Glycosylated cellobiohydrolase I catalytic domain complex with cellobiose and Cd+2 ions (yellow) (PDB code [[3cel]])'>
<StructureSection load='3cel' size='350' side='right' scene='48/488493/Cv/1' caption='Glycosylated cellobiohydrolase I catalytic domain complex with cellobiose and Cd+2 ions (PDB code [[3cel]])'>




== Function ==
== Function ==


'''Cellobiohydrolase''' (CBH) is a cellulase which degrades cellulose by hydrolysing the 1,4-β-D-glycosidic bonds.  CBH is an exocellulase which cleaves two to four units from the ends of cellulose.  There are two types of CBH.  '''CBHI''' cleaves progressively from the reducing end while '''CBHII''' cleaves progressively from the nonreducing end of cellulose.<ref>PMID:9466911</ref>. The exo-acting CBH I is called '''Cel7A'''. The endo-acting CBH I is called '''Cel7B'''.  
'''Cellobiohydrolase''' (CBH) is a cellulase which degrades [[cellulose]] by hydrolysing the 1,4-β-D-glycosidic bonds.  CBH is an exocellulase which cleaves two to four units from the ends of cellulose.  There are two types of CBH.  '''CBHI''' cleaves progressively from the reducing end while '''CBHII''' cleaves progressively from the nonreducing end of cellulose.<ref>PMID:9466911</ref>. The exo-acting CBH I is called '''Cel7A'''. The endo-acting CBH I is called '''Cel7B'''.  '''Cellobiohydrolase Cel6A''' contains an active site within a tunnel which opens and closes in response to ligand binding<ref>PMID:12842048</ref>.


==Structural highlights ==
==Structural highlights ==


The <scene name='48/488493/Cv/2'>active site</scene> of CBH contains <scene name='48/488493/Cv/3'>Glu-Asp-Glu</scene> (Gln-Asp-Glu in [[3cel]], cyan) and is situated at the <scene name='48/488493/Cv/4'>bottom of a tunnel</scene>.<ref>PMID:8951380</ref>
The <scene name='48/488493/Cv/6'>active site</scene> of CBH contains <scene name='48/488493/Cv/7'>Glu-Asp-Glu</scene> (Gln-Asp-Glu in [[3cel]], cyan) and is situated at the <scene name='48/488493/Cv/9'>bottom of a tunnel</scene>.<ref>PMID:8951380</ref> Water molecules shown as red spheres.
</StructureSection>
 
==3D structures of cellobiohydrolase==
==3D structures of cellobiohydrolase==
[[Cellobiohydrolase 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*Cellobiohydrolase I or exoglucanase 1
 
**[[1cel]], [[4p1h]], [[4p1j]] – HjCBHI Cel7A catalytic domain – ''Hypocrea jecorina''<BR />
**[[2cel]], [[4cel]], [[1egn]], [[1q2b]] - HjCBHI catalytic domain (mutant) <BR />
**[[1cbh]], [[2cbh]], [[5x34]], [[5x35]], [[5x36]], [[5x37]], [[5x38]] – HjCBHI C terminal – NMR<BR />
**[[5x3c]] – HjCBHI C terminal (mutant) – NMR<br />
**[[1azh]], [[1az6]], [[1azj]], [[1azk]] - HjCBHI cellulose-binding domain (mutant) - NMR<BR />
**[[1gpi]] - PcCBHI Cel7D catalytic domain – ''Phanerochaete chrysosporium''<BR />
**[[1q9h]], [[3pfj]] - TeCBHI Cel7A catalytic domain – ''Talaromyces emersonii''<BR />
**[[2yok]], [[2y9n]] - CBHI Cel7A catalytic domain – ''Hypocrea lixii''<br />
**[[5mcc]], [[5mcd]], [[5mce]], [[5mcf]], [[5mch]], [[5mci]], [[5mcj]], [[5mck]], [[5mcl]], [[5mcm]], [[5mcn]], [[4xnn]] – wfCBHI – water flea<br />
**[[4zzv]], [[5amp]] - GcCBHI Cel7A catalytic domain – ''Galactomyces candidum''<br />
**[[2mwk]], [[2mwj]] - TrCBHI Cel7A carbohydrate-binding module 1 domain – ''Trichoderma reesei''<br />
 
*''Cellobiohydrolase I binary complex''
 
**[[3cel]], [[4uwt]] - HjCBHI Cel7A catalytic domain (mutant) + cellobiose<br />
**[[5cel]], [[1q2e]], [[4d5v]] - HjCBHI Cel7A catalytic domain (mutant) + cellotetraose<br />
**[[4d5q]] - HjCBHI Cel7A catalytic domain + cellopentaose<br />
**[[6cel]], [[4d5o]], [[4d5p]] - HjCBHI Cel7A catalytic domain (mutant) + cellopentaose<br />
**[[7cel]] - HjCBHI catalytic domain (mutant) + glucose<br />
**[[2v3i]], [[2v3r]] - HjCBHI Cel7A catalytic domain + phenanthrenolol<br />
**[[1dy4]] - TrCBHI Cel7A catalytic domain + propranolol <BR />
**[[4c4c]] - TrCBHI Cel7A catalytic domain (mutant) + cellononaose <br />
**[[4c4d]], [[4d5i]], [[4d5j]] - TrCBHI Cel7A catalytic domain (mutant) + cellotrioside <br />
**[[1h46]] - PcCBHI Cel7D catalytic domain + propranolol<br />
**[[1z3t]] - PcCBHI Cel7D catalytic domain + cellobiose<br />
**[[1z3v]] - PcCBHI Cel7D catalytic domain + lactose<br />
**[[1z3w]] - PcCBHI Cel7D catalytic domain + cellobioimidazole<BR />
**[[3pfx]] - TeCBHI Cel7A catalytic domain + cellobiose<br />
**[[3pfz]] - TeCBHI Cel7A catalytic domain + cellotetraose + cellobiose<br />
**[[3pl3]] - TeCBHI Cel7A catalytic domain + cellopentaose<br />
**[[4zzu]] - GgCBHI Cel7A catalytic domain + cellotetraose – ''Galactomyces geotrichum''<br />
**[[4zzw]] - GgCBHI Cel7A catalytic domain + cellobiose <br />
**[[4zzt]] - GcCBHI Cel7A catalytic domain + cellotraiose <br />
 
*Cellobiohydrolase II or Cellobiohydrolase Cel6A or exoglucanase 2
 
**[[3cbh]] – HjCBHII core<BR />
**[[1cb2]] – HjCBHII catalytic domain (mutant) <BR />
**[[1hgw]], [[1hgy]], [[1oc6]] – HjCBHII Cel6A catalytic domain (mutant)
 
*''Cellobiohydrolase II complex''
 
**[[1bvw]] - HiCBHII catalytic domain + mannose – ''Humicola insolens''<BR />
**[[4au0]] - HjCBHII catalytic domain Cel6A (mutant) + cellobioside<br />
**[[2bvw]] - HiCBHII Cel6A catalytic domain + glucose + cellotetraose<br />
**[[1gz1]], [[1oc5]] - HiCBHII Cel6A catalytic domain (mutant) + cellotetraose<br />
**[[1oc7]] - HiCBHII Cel6A catalytic domain (mutant) + cellopentaose<br />
**[[1ocj]] - HiCBHII Cel6A catalytic domain + cellopentaose<br />
**[[1ocb]] - HiCBHII Cel6A catalytic domain + fluorescent substrate<BR />
**[[1ocn]] - HiCBHII Cel6A catalytic domain (mutant) + isofagomine<BR />
**[[1qjw]] - TrCBHII Cel6A catalytic domain (mutant) + cellotetraose<br />
**[[4ax6]], [[4ax7]] - TrCBHII Cel6A catalytic domain (mutant) + cellobioside<br />
**[[1qk0]] - TrCBHII Cel6A catalytic domain + polysaccharide<BR />
**[[1qk2]] - TrCBHII Cel6A catalytic domain + cellotetraose<br />
 
*Other cellobiohydrolases
 
**[[1l1y]] – CtCBH CelS – ''Clostridium thermocellum''<BR />
**[[1rq5]] - CtCBHA (mutant) <BR />
**[[1ut9]] - CtCBHA catalytic domain<BR />
**[[1sqj]] - GgCBH <BR />
**[[3a64]], [[3vog]] - CcCBH Cel6C catalytic domain - ''Coprinopsis cinerea''<BR />
**[[3vof]], [[3voj]] - CcCBHI Cel6C catalytic domain (mutant)<br />
**[[4v1z]] – AfCBH Cel7A – ''Aspergillus fumigatus''<br />
**[[2mte]] – CBH CelS residues 673-741 – ''Ruminiclostridium thermocellum'' - NMR<br />
 
*''Other cellobiohydrolases binary complex''
 
**[[1l2a]] - CtCBH CelS + glucose<br />
**[[2ebs]] – GgCBH (mutant) + heptasaccharide<BR />
**[[3a9b]], [[3voh]] - CcCBHI Cel6C catalytic domain + cellobiose<br />
**[[3abx]], [[3voi]] - CcCBHI Cel6C catalytic domain + cellotriose<br />
**[[4v20]] - AfCBH Cel7A + disaccharide<br />
**[[4v0z]] - TrCBH Cel7A + cellobiose<br />
**[[4vwt]] - TrCBH Cel7A (mutant) + cellobiose<br />


}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Karsten Theis
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