6hf6: Difference between revisions
New page: '''Unreleased structure''' The entry 6hf6 is ON HOLD Authors: Engilberge, S., Wagner, T., Santoni, G., Breyton, C., Shima, S., Franzetti, B., Riobe, F., Maury, O., Girard, E. Descripti... |
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==Crystal structure of the Protease 1 (E29A,E60A,E80A) from Pyrococcus horikoshii co-crystallized with Tb-Xo4.== | |||
<StructureSection load='6hf6' size='340' side='right'caption='[[6hf6]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6hf6]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HF6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HF6 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7MT:Tb-Xo4'>7MT</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PH1704 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hf6 OCA], [http://pdbe.org/6hf6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hf6 RCSB], [http://www.ebi.ac.uk/pdbsum/6hf6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hf6 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/DEGLY_PYRHO DEGLY_PYRHO]] Deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage (By similarity). Also displays proteolytic activity (PubMed:11114201).[UniProtKB:Q51732]<ref>PMID:11114201</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Obtaining crystals and solving the phase problem remain major hurdles encountered by bio-crystallographers in their race to obtain new high-quality structures. Both issues can be overcome by the crystallophore, Tb-Xo4, a lanthanide-based molecular complex with unique nucleating and phasing properties. This article presents examples of new crystallization conditions induced by the presence of Tb-Xo4. These new crystalline forms bypass crystal defects often encountered by crystallographers, such as low-resolution diffracting samples or crystals with twinning. Thanks to Tb-Xo4's high phasing power, the structure determination process is greatly facilitated and can be extended to serial crystallography approaches. | |||
Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.,Engilberge S, Wagner T, Santoni G, Breyton C, Shima S, Franzetti B, Riobe F, Maury O, Girard E J Appl Crystallogr. 2019 Jun 28;52(Pt 4):722-731. doi: 10.1107/S1600576719006381., eCollection 2019 Aug 1. PMID:31396026<ref>PMID:31396026</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6hf6" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pyrococcus horikoshii]] | |||
[[Category: Breyton, C]] | |||
[[Category: Engilberge, S]] | |||
[[Category: Franzetti, B]] | |||
[[Category: Girard, E]] | |||
[[Category: Maury, O]] | [[Category: Maury, O]] | ||
[[Category: Riobe, F]] | [[Category: Riobe, F]] | ||
[[Category: Santoni, G]] | [[Category: Santoni, G]] | ||
[[Category: | [[Category: Shima, S]] | ||
[[Category: Wagner, T]] | |||
[[Category: Cccluster]] | |||
[[Category: Crystallization]] | |||
[[Category: Crystallophore]] | |||
[[Category: De novo phasing]] | |||
[[Category: Hydrolase]] | |||
[[Category: Lanthanide complex]] | |||
[[Category: Meshandcollect]] | |||
[[Category: Multi-crystals data collection]] | |||
[[Category: Sad]] | |||
[[Category: Tb-xo4]] | |||