|
|
| (20 intermediate revisions by 3 users not shown) |
| Line 1: |
Line 1: |
| {{STRUCTURE_3uc1| PDB=3uc1 | SIZE=400| SCENE= |right|CAPTION=Gyrase type IIA subunit A C-terminal domain with Ca+2 (green), acetate and glycerol, [[3uc1]] }}
| | <StructureSection load='' size='350' side='right' caption='Gyrase subunit A N-terminal (cyan) and subunit B C-terminal (magenta) complex with DNA, inhibitor, sulfate and Mn+2 ion (purple) (PDB entry [[4plb]])' scene='41/410321/Cv/1'> |
| | ==Function== |
|
| |
|
| '''Gyrase (Gyr)''' is a type of topoisomerase II in prokaryotes which unwinds double stranded DNA. The DNA Gyr cutting allows the formation of a negative DNA supercoil which enables replication of DNA. Gyr consists of 2 subunits: GyrA and GyrB. Reverse gyrase (Top-RG) is a type of topoisomerase I which catalyses the formation of positive DNA supercoil. <ref>PMID:16397501</ref> | | '''Gyrase (Gyr)''' is a type of topoisomerase II in prokaryotes which unwinds double stranded DNA. The DNA Gyr cutting allows the formation of a negative DNA supercoil which enables replication of DNA<ref>PMID:1657531</ref> Gyr consists of 2 subunits: GyrA and GyrB. '''Reverse gyrase''' (Top-RG) is a type of topoisomerase I which catalyses the formation of positive DNA supercoil. <ref>PMID:15673717</ref> See also [[Isomerases]]. |
|
| |
|
| ==3D Structure of Gyrase== | | ==Relevance== |
|
| |
|
| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | GyrA inhibitor [[Ciprofloxacin]] is used as antibiotic drug. Fluoroquinolones are Gyr inhibitors used in treatment of multi drug-resistant tuberculosis<ref>PMID:15047530</ref> |
| {{#tree:id=OrganizedByTopic|openlevels=0|
| |
|
| |
|
| *Gyrase Subunit A
| | == Structural highlights == |
| | <scene name='41/410321/Cv/5'>Gyrase subunit A N-terminal and subunit B C-terminal complex with DNA and inhibitor</scene>. A <scene name='41/410321/Cv/6'>potential drug interacts with both DNA strands and in the hydrophobic pocket between subunits A and B</scene><ref>PMID:24900889</ref>. Water molecules shown as red spheres. |
|
| |
|
| **[[3l6v]] – GyrA C-terminal – ''Xanthomonas campestris'' <br />
| | ==3D Structure of Gyrase== |
| **[[2wl2]], [[2y3p]], [[4ckl]] – EcGyrA N-terminal+simocylinone – ''Escherichia coli''<br />
| | [[Gyrase 3D Structures]] |
| **[[1ajb]] - EcGyrA N-terminal+novobiocin<br />
| |
| **[[4ckk]] - EcGyrA N-terminal<br />
| |
| **[[1zi0]], [[1ab4]] - EcGyrA C-terminal<br />
| |
| **[[3ku8]] – EcGyrA fragment+CcdB<br />
| |
| **[[1x75]] – EcGyrA14+CcdB<br />
| |
| **[[3kua]], [[4elz]] - GyrA fragment+CcdB – ''Vibrio fischeri''<br />
| |
| **[[3ilw]], [[3ifz]] - MtGyrA ATPase domain – ''Mycobacterium tuberculosis''<br />
| |
| **[[3uc1]], [[4g3n]] - MtGyrA C-terminal<br />
| |
| **[[1suu]] - GyrA C-terminal – ''Borrelia burgdorferi''<br />
| |
| **[[3no0]] - GyrA C-terminal – ''Aquifex aeolicus''<br />
| |
| **[[3lpx]] – GyrA N-terminal – ''Colwellia psychrerithraea''<br />
| |
| **[[4ely]] – GyrA residues 363-497 + CcdB – ''Shigella flexneri''<br />
| |
| **[[4ddq]] – GyrA – ''Bacillus subtilis''<br />
| |
| | |
| *Gyrase Subunit B
| |
| | |
| **[[3g75]], [[3g7b]], [[3g7e]], [[3ttz]], [[3u2d]], [[3u2k]], [[4p8o]] – GyrB+ inhibitor – ''Staphylococcus aureus''<br />
| |
| **[[4urm]], [[4uro]] - SaGyrB N-terminal + antibiotic<br />
| |
| **[[2zjt]], [[3ig0]], [[3m4i]] - MtGyrB C-terminal<br />
| |
| **[[3cwv]] – GyrB truncated – ''Myxococcus xanthus''<br />
| |
| **[[4wub]], [[4wuc]], [[4wud]], [[4xtj]] - EcGyrB N-terminal<br />
| |
| **[[1kzn]], [[1ei1]], [[1aj6]] - EcGyrB N-terminal+ antibiotic<br />
| |
| **[[4duh]] - EcGyrB N-terminal+ inhibitor<br />
| |
| **[[4hyp]] - EcGyrB residues 15-220 + inhibitor<br />
| |
| **[[1kij]] – GyrB ATPase domain+ antibiotic – ''Thermus thermophilus''<br />
| |
| **[[4b6c]] - GyrB ATPase domain – ''Mycobacterium smegmatis''<br />
| |
| **[[4gee]], [[4gfn]], [[4ggl]], [[4hxw]], [[4k4o]], [[4kfg]], [[4ksg]], [[4ksh]], [[4ktn]] – GyrB + inhibitor – ''Enterococcus faecalis''<br />
| |
| **[[3zkb]], [[3zkd]] - MtGyrB ATPase domain + AMPPNP<br />
| |
| **[[3zm7]] - MtGyrB ATPase domain + AMPPCP<br />
| |
| **[[4bae]] - MtGyrB ATPase domain (mutant) + inhibitor<br />
| |
| | |
| *Gyrase Subunit A+Subunit B
| |
| | |
| **[[2xco]], [[2xcq]] - SaGyrB C-terminal/SaGyrA N-terminal <br />
| |
| **[[4plb]] - SaGyrB C-terminal/SaGyrA N-terminal +DNA<br />
| |
| **[[2xcr]], [[2xcs]], [[4bul]] - SaGyrB C-terminal/SaGyrA N-terminal (mutant)+DNA<br />
| |
| **[[2xct]] - SaGyrB C-terminal/SaGyrA N-terminal (mutant) +DNA+ antibiotic<br />
| |
| **[[4tma]] - SaGyrB C-terminal- + SaGyrA N-terminal + Gyr inhibitor YACG<br />
| |
| **[[3nuh]] – EcGyrA+EcGyrB
| |
|
| |
|
| *Reverse Gyrase
| | </StructureSection> |
|
| |
|
| **[[1gku]] – AfTop-RG – ''Archaeoglobus fulgidus''<br />
| |
| **[[1gl9]] - AfTop-RG+ADPNP<br />
| |
| **[[3oiy]], [[3p4y]] – TmTop-RG helicase domain – ''Thermotoga maritima''<br />
| |
| **[[4ddt]], [[4ddu]], [[4ddv]], [[4ddw]], [[4ddx]] - TmTop-RG
| |
| }}
| |
| ==Additional Resources== | | ==Additional Resources== |
| For additional information, see: [[Bacterial Infections]] | | For additional information, see: [[Bacterial Infections]] |