Colicin A: Difference between revisions
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Colicin A is a type of [[Colicin]], a bacteriocin made by <i>E. coli</i> which acts against other nearby <i>E. coli</i> to kill them by forming a pore in the membrane, leading to depolarisation of the membrane which kills the cell. | '''Colicin A''' is a type of [[Colicin]], a bacteriocin made by <i>E. coli</i> which acts against other nearby <i>E. coli</i> to kill them by forming a pore in the membrane, leading to depolarisation of the membrane which kills the cell. | ||
==Synthesis== | ==Synthesis== | ||
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Col A when present in an <i>E. coli</i> cell is able to affect macromolecular synthesis throughout the cell, affecting many of the processes in the cell. One such affected system is nucleic acid synthesis; it is halted very soon after Col A is added to the system<ref>PMID:4905544 </ref>. | Col A when present in an <i>E. coli</i> cell is able to affect macromolecular synthesis throughout the cell, affecting many of the processes in the cell. One such affected system is nucleic acid synthesis; it is halted very soon after Col A is added to the system<ref>PMID:4905544 </ref>. | ||
Colicin A also has a negative impact on the availability of ATP-dependent active transports, such as some permease activities, such as the uptake of labelled isoleucine<ref> PMID:4905544 </ref>. | Colicin A also has a negative impact on the availability of ATP-dependent active transports, such as some permease activities, such as the uptake of labelled isoleucine<ref> PMID:4905544 </ref>. | ||
==3D structures of colicin A== | |||
[[Colicin]] | |||
The structure determination of the C-terminal domain of protein was done by Dr. Parker and colleagues<ref> PMID:2909895 </ref><ref> PMID:1373773 </ref>. It enabled to better understand the stability of hydrophobic pockets, the energy transfer among the three tryptophan residue in that domain, and the creation of double-cysteine mutants to understand the transition from soluble to membrane associated protein<ref> PMID:8663026 </ref><ref> PMID:7849033 </ref><ref> PMID:7716161 </ref>. | |||
==References== | ==References== | ||
<references/> | <references/> | ||