User:Luis Netto/Sandbox 1: Difference between revisions

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==Ohr ==

Ohr (Organic Hydroperoxide Resistance Protein)[[1zb8]] is a Cys based peroxidase <ref>PMID: 12540833</ref>,<ref>PMID: 12485986</ref>

that display higher preference for hydrogen peroxide (H2O2) than for organic hydroperoxides. Accordingly, bacteria with the gene for Ohr deleted displayed increased sensitivity for organic hydroperoxides but not for hydrogen peroxide <ref>PMID: 9573147</ref>.

~~Ohr is a tight homo-dimer a [[Image:Ohr_black_white.png | thumb]]~~

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Ohr is a Cys based peroxidase that means the active site Cys is very reactive towards organic hydroperoxides. This high reactivity is achieved in the active site by interactions with fully conserved Arg and Glu residues among others

~~==Your Heading Here (maybe something like 'Structure')==~~

~~Anything in this section will appear adjacent to the 3D structure and will be scrollable.~~

~~</StructureSection>~~

~~It is involved in the response of~~

== Disease ==

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== Structural highlights ==

Ohr is a homo-dimer, with a symmetrical,oval shape. The two monomers are tightly wrapped around each other in a head-to-tail orientation to generate a compact quaternary structure (Figure 1).

[[Image:Homodimer_Ohr.png|thumb|Figure1 Ohr homodimer]]

In Figure 1, one monomer is depicted in light blue the other in green. Reactive Cys also called peroxidatic Cys (Cp) is depicted in orange. Catalytic Arg (red) and Glu (pink) compose the catalytic triad with Cp. The catalytic Glu orientates the catalytic Arg towards Cp, increasing its nucleophilicity.

Arg19 and Glu 50 of one chain (lets say chain A) compose the <scene name='78/785321/Catalytic_triad/1'>catalytic triad</scene> triad with Cys61 of the other chain (in this case chain B).

The structures of Ohr in the reduced and oxidized form were also obtained. For Ohr from Xylella fastidiosa the structures of both reduced and oxidized are available.

<scene name='78/785321/Oxidized_ohr/1'>Text To Be Displayed</scene>

~~</StructureSection>~~

== References ==

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 ==Ohr ==
-<StructureSection load='1ZB8' size='340' side='right' caption='Caption for this structure' scene=''>
+<StructureSection load='1ZB8' size='340' side='right' caption='Caption overall Ohr structure' scene=''>
 Ohr (Organic Hydroperoxide Resistance Protein)[[1zb8]] is a Cys based peroxidase <ref>PMID: 12540833</ref>,<ref>PMID: 12485986</ref>
 that display higher preference for hydrogen peroxide (H2O2) than for organic hydroperoxides. Accordingly, bacteria with the gene for Ohr deleted displayed increased sensitivity for organic hydroperoxides but not for hydrogen peroxide <ref>PMID: 9573147</ref>.
-Ohr is a tight homo-dimer a  [[Image:Ohr_black_white.png | thumb]]
@@ Line 22: / Line 11: @@
 Ohr is a Cys based peroxidase that means the active site Cys is very reactive towards organic hydroperoxides. This high reactivity is achieved in the active site by interactions with fully conserved Arg and Glu residues among others
-==Your Heading Here (maybe something like 'Structure')==
-Anything in this section will appear adjacent to the 3D structure and will be scrollable.
-</StructureSection>
-It is involved in the response of
 == Disease ==
@@ Line 54: / Line 34: @@
 == Structural highlights ==
+Ohr is a homo-dimer, with a symmetrical,oval shape. The two monomers are tightly wrapped around each other in a head-to-tail orientation to generate a compact quaternary structure (Figure 1).
+[[Image:Homodimer_Ohr.png|thumb|Figure1 Ohr homodimer]]
+In Figure 1, one monomer is depicted in light blue the other in green. Reactive Cys also called peroxidatic Cys (Cp) is depicted in orange. Catalytic Arg (red) and Glu (pink) compose the catalytic triad with Cp. The catalytic Glu orientates the catalytic Arg towards Cp, increasing its nucleophilicity.
 Arg19 and Glu 50 of one chain (lets say chain A) compose the  <scene name='78/785321/Catalytic_triad/1'>catalytic triad</scene> triad with Cys61 of the other chain (in this case chain  B).
+The structures of Ohr in the reduced and oxidized form were also obtained. For Ohr from Xylella fastidiosa the structures of both reduced and oxidized  are available.
+ <scene name='78/785321/Oxidized_ohr/1'>Text To Be Displayed</scene>
-</StructureSection>
 == References ==
 <references/>