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| <StructureSection load='3ovs' size='450' side='right' caption='Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, sulfate, ethanediol and Ca+2 ion (green) (PDB entry [[3ovs]])' scene='55/551188/Cv/1'> | | <StructureSection load='3ovs' size='350' side='right' caption='Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, sulfate, ethanediol and Ca+2 ion (green) (PDB entry [[3ovs]])' scene='55/551188/Cv/1'> |
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| '''CCA-adding enzyme''' (CAE) builds or repairs the trinucleotice CCA at the 3’ end of tRNA. CAE has one active site which is capable of binding CTP and ATP in a specific sequence. In the bacteria Aquifex aeolicus, CAE consists of 2 subunits. One specific for CTP and the other for ATP. CAE builds the CCA without a genetic template.<ref>PMID:15295590</ref> | | '''CCA-adding enzyme''' (CAE) builds or repairs the trinucleotice CCA at the 3’ end of tRNA. CAE has one active site which is capable of binding CTP and ATP in a specific sequence. In the bacteria Aquifex aeolicus, CAE consists of 2 subunits. One specific for CTP and the other for ATP. CAE builds the CCA without a genetic template.<ref>PMID:15295590</ref> |
| *<scene name='55/551188/Cv/2'>Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, ethanediol and Ca+2 ion</scene> (PDB entry [[3ovs]]). | | *<scene name='55/551188/Cv/4'>CCA-adding enzyme complex with tRNA, CTP, ethanediol and Ca+2 ion</scene> (PDB entry [[3ovs]]). |
| *<scene name='55/551188/Cv/3'>Binding site of CCA-adding enzyme</scene> (PDB entry [[3ovs]]).<ref>PMID:21071662</ref> | | *<scene name='55/551188/Cv/5'>Ca+2 coordination site of CCA-adding enzyme</scene> (PDB entry [[3ovs]]).<ref>PMID:21071662</ref> |
| </StructureSection>
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| ==3D structures of CCA-adding enzyme== | | ==3D structures of CCA-adding enzyme== |
| | [[CCA-adding enzyme 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *CCA-adding enzyme
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| **[[1miv]] – BsCAE – ''Bacillus stearothermophilus''<BR />
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| **[[1uet]], [[1r8a]] – AfCAE – ''Archaeoglobus fulgidus''<BR />
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| **[[1ou5]], [[4x4w]] – hCAE – human<br />
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| *CCA-adding enzyme binary complex with nucleotide
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| **[[1miw]] – BsCAE + ATP<BR />
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| **[[1uev]], [[1r8b]] – AfCAE + ATP<BR />
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| **[[1miy]] – BsCAE + CTP<BR />
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| **[[1ueu]], [[1r89]] – AfCAE + CTP<BR />
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| **[[1r8c]] – AfCAE + UTP<BR />
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| *CCA-adding enzyme binary complex with tRNA
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| **[[2dr5]] – AfCAE + tRNA 32-mer<BR />
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| **[[2dr7]], [[2zh1]], [[2zh7]], [[2zh9]], [[4x4q]], [[4x4t]], [[4x4u]] – AfCAE + tRNA 33-mer<BR />
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| **[[2dr9]], [[2zh2]], [[2zh3]], [[2zh4]], [[2zh5]], [[2zh8]], [[2zhb]] – AfCAE + tRNA 34-mer<BR />
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| **[[2drb]], [[3ouy]], [[4x4p]] – AfCAE + tRNA 35-mer<BR />
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| *CCA-adding enzyme ternary complex with nucleotide and tRNA
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| **[[1sz1]] – AfCAE + GTP + UMP + CMP + tRNA<BR />
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| **[[1tfw]] – AfCAE + ATP + tRNA 3’ end<BR />
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| **[[1tfy]] – AfCAE + CTP + tRNA 3’ end<BR />
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| **[[2dr8]], [[2zha]], [[4x4s]] – AfCAE + CTP + tRNA 33-mer<BR />
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| **[[4x4r]], [[4x4v]] – AfCAE + AMPCPP + tRNA 33-mer<BR />
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| **[[4x4n]] – AfCAE + GTP + tRNA 33-mer<BR />
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| **[[2dra]], [[2zh6]], [[3ov7]], [[4x4o]] – AfCAE + ATP + tRNA 34-mer<BR />
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| **[[3ova]] – AfCAE + ATP derivative + tRNA 34-mer<BR />
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| **[[2dvi]], [[3ovs]] – AfCAE + CTP + tRNA 34-mer<BR />
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| **[[3ovb]] – AfCAE + ATP + tRNA 35-mer<BR />
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |