6gmf: Difference between revisions
New page: '''Unreleased structure''' The entry 6gmf is ON HOLD until Paper Publication Authors: Grogan, G., Dubiel, P., Sharma, M., Klenk, J., Hauer, B. Description: Structure of Cytochrome P450... |
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==Structure of Cytochrome P450 CYP109Q5 from Chondromyces apiculatus== | |||
<StructureSection load='6gmf' size='340' side='right'caption='[[6gmf]], [[Resolution|resolution]] 1.55Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6gmf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GMF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GMF FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gmf OCA], [http://pdbe.org/6gmf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gmf RCSB], [http://www.ebi.ac.uk/pdbsum/6gmf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gmf ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
One of the major challenges in chemical synthesis is the selective oxyfunctionalization of non-activated C-H bonds, which can be enabled by biocatalysis using cytochrome P450 monooxygenases. In this study, we report on the characterization of the versatile CYP109Q5 from Chondromyces apiculatus DSM436, which is able to functionalize a wide range of substrates (terpenes, steroids and drugs), including the ring of beta-ionone in non-allylic positions. The crystal structure of CYP109Q5 revealed flexibility within the active site pocket that permitted the accommodation of bulky substrates, and enabled a structure-guided approach to engineering the enzyme. Some variants of CYP109Q5 displayed a switch in selectivity towards the non-allylic positions of beta-ionone, allowing the simultaneous production of 2- and 3-hydroxy-beta-ionone, which are chemically challenging to synthesize and are important precursors for carotenoid synthesis. An efficient whole-cell system finally enabled the production of up to 0.5 g l(-1) hydroxylated products of beta-ionone; this system can be applied to product identification in further biotransformations. Overall, CYP109Q5 proved to be highly evolvable and active. The studies in this work demonstrate that, using rational mutagenesis, the highly versatile CYP109Q5 generalist can be progressively evolved to be an industrially valuable specialist for the synthesis of specific products. | |||
Characterization and structure-guided engineering of the novel versatile terpene monooxygenase CYP109Q5 from Chondromyces apiculatus DSM436.,Klenk JM, Dubiel P, Sharma M, Grogan G, Hauer B Microb Biotechnol. 2019 Mar;12(2):377-391. doi: 10.1111/1751-7915.13354. Epub, 2018 Dec 27. PMID:30592153<ref>PMID:30592153</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6gmf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Dubiel, P]] | |||
[[Category: Grogan, G]] | |||
[[Category: Hauer, B]] | [[Category: Hauer, B]] | ||
[[Category: Klenk, J]] | [[Category: Klenk, J]] | ||
[[Category: Sharma, M]] | [[Category: Sharma, M]] | ||
[[Category: | [[Category: Biocatalysis]] | ||
[[Category: Oxidoreductase]] | |||
[[Category: P450]] | |||
[[Category: Terpene]] | |||
Latest revision as of 09:36, 27 March 2019
Structure of Cytochrome P450 CYP109Q5 from Chondromyces apiculatusStructure of Cytochrome P450 CYP109Q5 from Chondromyces apiculatus
Structural highlights
Publication Abstract from PubMedOne of the major challenges in chemical synthesis is the selective oxyfunctionalization of non-activated C-H bonds, which can be enabled by biocatalysis using cytochrome P450 monooxygenases. In this study, we report on the characterization of the versatile CYP109Q5 from Chondromyces apiculatus DSM436, which is able to functionalize a wide range of substrates (terpenes, steroids and drugs), including the ring of beta-ionone in non-allylic positions. The crystal structure of CYP109Q5 revealed flexibility within the active site pocket that permitted the accommodation of bulky substrates, and enabled a structure-guided approach to engineering the enzyme. Some variants of CYP109Q5 displayed a switch in selectivity towards the non-allylic positions of beta-ionone, allowing the simultaneous production of 2- and 3-hydroxy-beta-ionone, which are chemically challenging to synthesize and are important precursors for carotenoid synthesis. An efficient whole-cell system finally enabled the production of up to 0.5 g l(-1) hydroxylated products of beta-ionone; this system can be applied to product identification in further biotransformations. Overall, CYP109Q5 proved to be highly evolvable and active. The studies in this work demonstrate that, using rational mutagenesis, the highly versatile CYP109Q5 generalist can be progressively evolved to be an industrially valuable specialist for the synthesis of specific products. Characterization and structure-guided engineering of the novel versatile terpene monooxygenase CYP109Q5 from Chondromyces apiculatus DSM436.,Klenk JM, Dubiel P, Sharma M, Grogan G, Hauer B Microb Biotechnol. 2019 Mar;12(2):377-391. doi: 10.1111/1751-7915.13354. Epub, 2018 Dec 27. PMID:30592153[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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