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| <StructureSection load='1raa' size='340' side='right' caption='Structure of E. coli aspartate carbamoyltransferase catalytic (grey and pink) and regulatory (green and yellow) subunits complex with CTP (stick figure) and Zn+2 (grey) (PDB code [[1raa]]).' scene=''> | | <StructureSection load='' size='350' side='right' caption='Structure of E. coli aspartate carbamoyltransferase catalytic (cyan and pink) and regulatory (green and yellow) subunits complex with inhibitor PALA and Zn+2 ions (grey) (PDB code [[1d09]]).' scene='59/592660/Cv/15'> |
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| == Function == | | == Function == |
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| '''Aspartate carbamoyltransferase''' (ATC) is part of the pyrimidine biosynthesis pathway. ATC catalyzes the condensation of aspartate and carbamoyl phosphate to N-carbamyl-L-aspartate and phosphate. ATC is composed of 2 trimers of catalytic subunits (C) and 3 dimers of regulatory subunits (R). The catalytic subunit contains an aspartate-binding domain and a carbamoyl-phosphate-binding domain. The regulatory subunit contains a nucleotide effectors-binding domain and a zinc domain. The Zn atom is essential for the association of the subunits. Binding of the substrate to the catalytic subunits results in a high-affinity state while binding of CTP to the regulatory subunit results in a low-affinity state. Malate and phosphonoacetyl-L-aspartate are inhibitors of ATC. | | '''Aspartate carbamoyltransferase''' (ATC) is part of the pyrimidine biosynthesis pathway. ATC catalyzes the condensation of aspartate and carbamoyl phosphate to N-carbamyl-L-aspartate and phosphate. The Zn atom is essential for the association of the subunits. Binding of the substrate to the catalytic subunits results in a high-affinity state while binding of CTP to the regulatory subunit results in a low-affinity state. Malate and phosphonoacetyl-L-aspartate (PALA) are inhibitors of ATC. For additional details see [[Aspartate Transcarbamoylase (ATCase)]]. |
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| == Disease == | | == Structural highlights == |
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| == Relevance ==
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| == Structural highlights == | | ATC is composed of <scene name='59/592660/Cv/10'>2 trimers of catalytic subunits (C) and 3 dimers of regulatory subunits (R)</scene>. Click to see <scene name='59/592660/Cv/11'>two catalytic trimers</scene> and <scene name='59/592660/Cv/12'>three regulatory dimers</scene>. The <scene name='59/592660/Cv/13'>catalytic subunit</scene> contains an <scene name='59/592660/Cv/14'>aspartate-binding domain</scene> and a carbamoyl-phosphate-binding domain. The regulatory subunit contains a nucleotide effectors-binding domain and a zinc domain. <ref>PMID:10651286</ref> |
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| ==3D structures of aspartate carbamoyltransferase== | | ==3D structures of aspartate carbamoyltransferase== |
| | [[Aspartate carbamoyltransferase 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
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| ===ATC===
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| [[2atc]], [[3at1]], [[6at1]], [[1pg5]], [[3d7s]] – EcATC C+R subunits – ''Escherichia coli''<br />
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| [[9atc]] – EcATC C (mutant) + R (mutant) subunits <br />
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| [[1ezz]], [[4e2f]] – EcATC C (mutant) + R subunits <br />
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| [[2qg9]], [[2qgf]] – EcATC C + R (mutant) subunits <br />
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| [[2be7]] – ATC C (mutant) + R subunits – ''Moritella profunda'' <br />
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| ===ATC binary complex===
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| [[1at1]] – EcATC C+R subunits + malonate<br />
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| [[1sku]] – EcATC C (mutant) +R subunits + malonate<br />
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| [[1r0b]] – EcATC C+R subunits + citrate<br />
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| [[1r0c]] – EcATC C+R subunits + N-carbamoyl-L-aspartate + phosphate<br />
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| [[1xjw]] – EcATC C (mutant) +R subunits + phosphonacetyl-L-aspartate <br />
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| [[1sku]] – EcATC C (mutant) +R subunits + phosphonoacetamide<br />
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| [[1tu0]] – EcATC C+R subunits + maltose<br />
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| [[4at1]] – EcATC C+R subunits + ATP<br />
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| [[5at1]], [[1raa]], [[1rab]], [[1rac]], [[1rad]], [[1rae]], [[1raf]], [[1rag]], [[1rah]], [[1rai]], [[1za1]], [[4fyw]] – EcATC C+R subunits + CTP<br />
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| [[8atc]], [[1acm]], [[1d09]], [[1q95]] – EcATC C+R subunits + phosphonacetyl aspartate<br />
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| [[1f1b]], [[1i5o]], [[1tth]] – EcATC C (mutant) +R subunits + phosphonacetyl aspartate<br />
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| [[2h3e]] – EcATC C+R subunits + phosphonacetyl asparagine<br />
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| [[1nbe]] – EcATC C (mutant) + R (mutant) subunits + malate<br />
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| [[2a0f]] – EcATC C (mutant) +R subunits + phosphonoacetamide<br />
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| [[3mpu]] – EcATC C (mutant) +R subunits + phosphate<br />
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| [[2be9]] – ATC C+R subunits + CTP – ''Sulfolobus acidocaldarius''<br />
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| [[2yww]] – ATC R subunit + ATP – ''Methanocaldococcus jannaschii''<br />
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| ===ATC ternary complex===
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| [[7at1]] – EcATC C+R subunits + ATP + maltose<br />
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| [[8at1]] – EcATC C+R subunits + CTP + maltose<br />
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| [[4fyv]] – EcATC C+R subunits + dCTP + phosphate<br />
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| [[4fyx]] – EcATC C+R subunits + dCTP + UTP<br />
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| [[4fyy]] – EcATC C+R subunits + CTP + UTP<br />
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| [[1za2]] – EcATC C+R subunits + CTP + phosphoric monoformamide ester<br />
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| [[2air]] – EcATC C+R subunits + L-alanosine + phosphoric monoformamide ester<br />
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| [[2fzc]], [[2fzg]] – EcATC C+R subunits + CTP + phosphonic acid derivative<br />
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| [[2fzk]] – EcATC C+R subunits + CTP + phosphonacetyl benzoate<br />
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| [[4f04]], [[4kgv]], [[4kgx]], [[4kgz]], [[4kh0]] – EcATC C+R subunits + nucleotide + phosphonacetyl aspartate<br />
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| [[2h3e]] – EcATC C+R subunits + phosphonacetyl asparagine + malate<br />
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| ===ATC quaternary complex===
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| [[1tug]] – EcATC C (mutant) +R subunits + phosphate + aspartate + phosphonoacetamide<br />
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| [[1tug]] – EcATC C (mutant) +R subunits + CTP + malonate + phosphonoacetamide<br />
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| [[4kh1]] – EcATC C+R subunits + UTP + CTP + phosphate + phosphonacetyl aspartate<br />
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| == References == | | == References == |