6hoj: Difference between revisions
New page: '''Unreleased structure''' The entry 6hoj is ON HOLD Authors: Mouilleron, S., Birgisdottir, A.B., Bhujbal, Z., Wirth, M., Sjottem, E., Evjen, G., Zhang, W., Lee, R., O'Reilly, N., Tooze... |
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==Structure of Beclin1 LIR motif bound to GABARAP== | |||
<StructureSection load='6hoj' size='340' side='right'caption='[[6hoj]], [[Resolution|resolution]] 1.51Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6hoj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HOJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HOJ FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GABARAP, FLC3B, HT004 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hoj OCA], [http://pdbe.org/6hoj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hoj RCSB], [http://www.ebi.ac.uk/pdbsum/6hoj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hoj ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/BECN1_HUMAN BECN1_HUMAN]] Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.<ref>PMID:20208530</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1). The latter contains PIK3C3/VPS34, PIK3R4/VPS15, BECN1/Beclin 1 and ATG14 and phosphorylates phosphatidylinositol to generate phosphatidylinositol 3-phosphate (PtdIns3P). Here, we show that PIK3C3, BECN1 and ATG14 contain functional LIR motifs and interact with the Atg8-family proteins with a preference for GABARAP and GABARAPL1. High resolution crystal structures of the functional LIR motifs of these core components of PtdIns3K-C1were obtained. Variation in hydrophobic pocket 2 (HP2) may explain the specificity for the GABARAP family. Mutation of the LIR motif in ATG14 did not prevent formation of the PtdIns3K-C1 complex, but blocked colocalization with MAP1LC3B/LC3B and impaired mitophagy. The ULK-mediated phosphorylation of S29 in ATG14 was strongly dependent on a functional LIR motif in ATG14. GABARAP-preferring LIR motifs in PIK3C3, BECN1 and ATG14 may, via coincidence detection, contribute to scaffolding of PtdIns3K-C1 on membranes for efficient autophagosome formation. | |||
Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs.,Birgisdottir AB, Mouilleron S, Bhujabal Z, Wirth M, Sjottem E, Evjen G, Zhang W, Lee R, O'Reilly N, Tooze SA, Lamark T, Johansen T Autophagy. 2019 Feb 15. doi: 10.1080/15548627.2019.1581009. PMID:30767700<ref>PMID:30767700</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 6hoj" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Human]] | |||
[[Category: Large Structures]] | |||
[[Category: Bhujbal, Z]] | [[Category: Bhujbal, Z]] | ||
[[Category: | [[Category: Birgisdottir, A B]] | ||
[[Category: Evjen, G]] | [[Category: Evjen, G]] | ||
[[Category: Johansen, T]] | [[Category: Johansen, T]] | ||
[[Category: Lamark, T]] | |||
[[Category: Lee, R]] | |||
[[Category: Mouilleron, S]] | |||
[[Category: Reilly, N O]] | |||
[[Category: Sjottem, E]] | [[Category: Sjottem, E]] | ||
[[Category: | [[Category: Tooze, S]] | ||
[[Category: Wirth, M]] | |||
[[Category: Zhang, W]] | [[Category: Zhang, W]] | ||
[[Category: | [[Category: Atg8]] | ||
[[Category: | [[Category: Autophagy]] | ||
[[Category: Lir]] | |||
[[Category: Signaling protein]] | |||