Flagellar filament of bacteria: Difference between revisions
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==R vs. L forms== | ==R vs. L forms== | ||
Quoted from <ref name="3a5x" />: | Quoted from Maki-Yonekura, Yonekura and Namba, 2010<ref name="3a5x" />: | ||
<blockquote> | <blockquote> | ||
"The bacterial flagellar filament is a helical propeller rotated by the flagellar motor for bacterial locomotion. The filament is a supercoiled assembly of a single protein, flagellin, and is formed by 11 protofilaments. For bacterial taxis, the reversal of motor rotation switches the supercoil between left- and right-handed, both of which arise from combinations of two distinct conformations and packing interactions of the L-type and R-type protofilaments."<ref name="3a5x" /> | "The bacterial flagellar filament is a helical propeller rotated by the flagellar motor for bacterial locomotion. The filament is a supercoiled assembly of a single protein, flagellin, and is formed by 11 protofilaments. For bacterial taxis, the reversal of motor rotation switches the supercoil between left- and right-handed, both of which arise from combinations of two distinct conformations and packing interactions of the L-type and R-type protofilaments."<ref name="3a5x" /> | ||
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The structure of a straight R form of the flagellar filament was reported in 2003<ref name="1ucu">PMID: 12904785</ref>, by fitting and extending a crystallographic model of the monomer ([[1io1]])<ref name="1io1">PMID: 11268201</ref> into an electron cryomicroscopic density map with resolution approaching 4 Å. The resulting full-length R form monomer, [[1ucu]], included terminal alpha helices that were absent in the crystallographic model. | The structure of a straight R form of the flagellar filament was reported in 2003<ref name="1ucu">PMID: 12904785</ref>, by fitting and extending a crystallographic model of the monomer ([[1io1]])<ref name="1io1">PMID: 11268201</ref> into an electron cryomicroscopic density map with resolution approaching 4 Å. The resulting full-length R form monomer, [[1ucu]], included terminal alpha helices that were absent in the crystallographic model. | ||
The structure of a straight L form of the flagellar filament was reported in 2010<ref name="3a5x">Maki-Yonekura, S., K. Yonekura, & K. Namba, 'Conformational change of flagellin for polymorphic supercoiling of the flagellar filament', Nat. Struct. Mol. Biol. 17, 417–422 (2010). [http://dx.doi.org/10.1038/nsmb.1774 doi:10.1038/nsmb.1774]</ref>. The monomer, [[3a5x]], was obtained by fitting the R-form monomer [[1ucu]] into the electron cryomicroscopy density map. | The structure of a straight L form of the flagellar filament was reported in 2010<ref name="3a5x">Maki-Yonekura, S., K. Yonekura, & K. Namba, 'Conformational change of flagellin for polymorphic supercoiling of the flagellar filament', Nat. Struct. Mol. Biol. 17, 417–422 (2010). [http://dx.doi.org/10.1038/nsmb.1774 doi:10.1038/nsmb.1774]</ref>. The monomer, [[3a5x]], was obtained by fitting the R-form monomer [[1ucu]] into the L form electron cryomicroscopy density map. | ||
===R to L Morphs=== | ===R to L Morphs=== | ||
<Structure size='450' frame='true' align='right' caption='' scene='Flagellar_filament_of_bacteria/Yale_morph_r_to_l/3' /> | <Structure size='450' frame='true' align='right' caption='' scene='Flagellar_filament_of_bacteria/Yale_morph_r_to_l/3' /> | ||
Below are [[morphs]] between the R and L forms of the monomer ([[1ucu]] and [[3a5x]] respectively) and the | Below are [[morphs]] between the R and L forms of the monomer ([[1ucu]] and [[3a5x]] respectively) and the filament. Filament models were kindly provided by T. Fujii and K. Namba. | ||
*R to L Monomer Morph (all residues aligned) (<scene name='Flagellar_filament_of_bacteria/Yale_morph_r_to_l/3'>restore initial scene</scene>)<ref>Chemically possible morph generated by the [http://molmovdb.org Yale Morph Server (molmovdb.org)], [http://www.molmovdb.org/cgi-bin/morph.cgi?ID=076723-32427 Job 076723-32427], file [[Image:1ucu-3a5x-yale-morph.pdb.gz]].</ref> | *R to L Monomer Morph (all residues aligned) (<scene name='Flagellar_filament_of_bacteria/Yale_morph_r_to_l/3'>restore initial scene</scene>)<ref>Chemically possible morph generated by the [http://molmovdb.org Yale Morph Server (molmovdb.org)], [http://www.molmovdb.org/cgi-bin/morph.cgi?ID=076723-32427 Job 076723-32427], file [[Image:1ucu-3a5x-yale-morph.pdb.gz]].</ref> | ||
Latest revision as of 14:02, 13 January 2019
R vs. L formsR vs. L forms
Quoted from Maki-Yonekura, Yonekura and Namba, 2010[1]:
"The bacterial flagellar filament is a helical propeller rotated by the flagellar motor for bacterial locomotion. The filament is a supercoiled assembly of a single protein, flagellin, and is formed by 11 protofilaments. For bacterial taxis, the reversal of motor rotation switches the supercoil between left- and right-handed, both of which arise from combinations of two distinct conformations and packing interactions of the L-type and R-type protofilaments."[1]
The structure of a straight R form of the flagellar filament was reported in 2003[2], by fitting and extending a crystallographic model of the monomer (1io1)[3] into an electron cryomicroscopic density map with resolution approaching 4 Å. The resulting full-length R form monomer, 1ucu, included terminal alpha helices that were absent in the crystallographic model.
The structure of a straight L form of the flagellar filament was reported in 2010[1]. The monomer, 3a5x, was obtained by fitting the R-form monomer 1ucu into the L form electron cryomicroscopy density map.
R to L MorphsR to L Morphs
|
Below are morphs between the R and L forms of the monomer (1ucu and 3a5x respectively) and the filament. Filament models were kindly provided by T. Fujii and K. Namba.
- R to L Monomer Morph (all residues aligned) ()[4]
- (Filament morphs are in preparation. Eric Martz 07:10, 15 May 2011 (IDT))
NotesNotes
- ↑ 1.0 1.1 1.2 Maki-Yonekura, S., K. Yonekura, & K. Namba, 'Conformational change of flagellin for polymorphic supercoiling of the flagellar filament', Nat. Struct. Mol. Biol. 17, 417–422 (2010). doi:10.1038/nsmb.1774
- ↑ Yonekura K, Maki-Yonekura S, Namba K. Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy. Nature. 2003 Aug 7;424(6949):643-50. PMID:12904785 doi:http://dx.doi.org/10.1038/nature01830
- ↑ Samatey FA, Imada K, Nagashima S, Vonderviszt F, Kumasaka T, Yamamoto M, Namba K. Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling. Nature. 2001 Mar 15;410(6826):331-7. PMID:11268201 doi:10.1038/35066504
- ↑ Chemically possible morph generated by the Yale Morph Server (molmovdb.org), Job 076723-32427, file File:1ucu-3a5x-yale-morph.pdb.gz.
- ↑ Linear interpolation morph, file File:1ucu 3a5x linmorph.pdb.gz. See Morphs.