Flagellar filament of bacteria: Difference between revisions
Jump to navigation
Jump to search
Eric Martz (talk | contribs) |
Michal Harel (talk | contribs) No edit summary |
||
| (18 intermediate revisions by one other user not shown) | |||
| Line 2: | Line 2: | ||
==R vs. L forms== | ==R vs. L forms== | ||
< | Quoted from Maki-Yonekura, Yonekura and Namba, 2010<ref name="3a5x" />: | ||
<blockquote> | |||
"The bacterial flagellar filament is a helical propeller rotated by the flagellar motor for bacterial locomotion. The filament is a supercoiled assembly of a single protein, flagellin, and is formed by 11 protofilaments. For bacterial taxis, the reversal of motor rotation switches the supercoil between left- and right-handed, both of which arise from combinations of two distinct conformations and packing interactions of the L-type and R-type protofilaments."<ref name="3a5x" /> | |||
</blockquote> | |||
The R form of the flagellar filament was | The structure of a straight R form of the flagellar filament was reported in 2003<ref name="1ucu">PMID: 12904785</ref>, by fitting and extending a crystallographic model of the monomer ([[1io1]])<ref name="1io1">PMID: 11268201</ref> into an electron cryomicroscopic density map with resolution approaching 4 Å. The resulting full-length R form monomer, [[1ucu]], included terminal alpha helices that were absent in the crystallographic model. | ||
The structure of a straight L form of the flagellar filament was reported in 2010<ref name="3a5x">Maki-Yonekura, S., K. Yonekura, & K. Namba, 'Conformational change of flagellin for polymorphic supercoiling of the flagellar filament', Nat. Struct. Mol. Biol. 17, 417–422 (2010). [http://dx.doi.org/10.1038/nsmb.1774 doi:10.1038/nsmb.1774]</ref>. The monomer, [[3a5x]], was obtained by fitting the R-form monomer [[1ucu]] into the L form electron cryomicroscopy density map. | |||
===R to L Morphs=== | |||
<Structure size='450' frame='true' align='right' caption='' scene='Flagellar_filament_of_bacteria/Yale_morph_r_to_l/3' /> | |||
*<scene name='Flagellar_filament_of_bacteria/Yale_morph_r_to_l/ | Below are [[morphs]] between the R and L forms of the monomer ([[1ucu]] and [[3a5x]] respectively) and the filament. Filament models were kindly provided by T. Fujii and K. Namba. | ||
*R to L Monomer Morph (all residues aligned) (<scene name='Flagellar_filament_of_bacteria/Yale_morph_r_to_l/3'>restore initial scene</scene>)<ref>Chemically possible morph generated by the [http://molmovdb.org Yale Morph Server (molmovdb.org)], [http://www.molmovdb.org/cgi-bin/morph.cgi?ID=076723-32427 Job 076723-32427], file [[Image:1ucu-3a5x-yale-morph.pdb.gz]].</ref> | |||
*<scene name='Flagellar_filament_of_bacteria/Linear_interp_morph_r_to_l/1'>R to L Monomer Morph (terminal helices aligned)</scene><ref>Linear interpolation morph, file [[Image:1ucu 3a5x linmorph.pdb.gz]]. See [[Morphs]].</ref> | |||
*(Filament morphs are in preparation. [[User:Eric Martz|Eric Martz]] 07:10, 15 May 2011 (IDT)) | |||
==Notes== | ==Notes== | ||
<references /> | <references /> | ||
==See Also== | |||
* | |||