6hct: Difference between revisions
New page: '''Unreleased structure''' The entry 6hct is ON HOLD Authors: Description: Category: Unreleased Structures |
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The | ==Crystal structure of Archeoglobus fulgidus L7Ae bound to its cognate UTR k-turn== | ||
<StructureSection load='6hct' size='340' side='right' caption='[[6hct]], [[Resolution|resolution]] 3.09Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6hct]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Arcfu Arcfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HCT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HCT FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpl7ae, AF_0764 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hct OCA], [http://pdbe.org/6hct PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hct RCSB], [http://www.ebi.ac.uk/pdbsum/6hct PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hct ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/RL7A_ARCFU RL7A_ARCFU]] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA and box C/D sRNAs.<ref>PMID:12560482</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A recent study has shown that archaeal L7Ae binds to a putative k-turn structure in the 5'-leader of the mRNA of its structural gene to regulate translation. To function as a regulator the RNA should be unstructured in the absence of protein, but it should adopt a k-turn-containing stem-loop on binding L7Ae. Sequence analysis of UTR sequences indicates that their k-turn elements will be unable to fold in the absence of L7Ae, and we have demonstrated this experimentally in solution using FRET for the Archeoglobus fulgidus sequence. We have solved the X-ray crystal structure of the complex of the A. fulgidus RNA bound to its cognate L7Ae protein. The RNA adopts a standard k-turn conformation, that is specifically recognized by the L7Ae protein so stabilizing the stem-loop. In-line probing of the natural-sequence UTR shows that the RNA is unstructured in the absence of L7Ae binding, but folds on binding the protein such that the ribosome binding site is occluded. Thus L7Ae regulates its own translation by switching the conformation of the RNA to alter accessibility. | |||
The role of RNA structure in translational regulation by L7Ae protein in archaea.,Huang L, Ashraf S, Lilley DMJ RNA. 2018 Oct 16. pii: rna.068510.118. doi: 10.1261/rna.068510.118. PMID:30327333<ref>PMID:30327333</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6hct" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Arcfu]] | |||
[[Category: Huang, L]] | |||
[[Category: Lilley, D M.J]] | |||
[[Category: Gene regulation]] | |||
[[Category: Kink-turn]] | |||
[[Category: Rna]] | |||
[[Category: Rna structure]] | |||
[[Category: X-ray crystallography]] | |||
Latest revision as of 11:44, 26 December 2018
Crystal structure of Archeoglobus fulgidus L7Ae bound to its cognate UTR k-turnCrystal structure of Archeoglobus fulgidus L7Ae bound to its cognate UTR k-turn
Structural highlights
Function[RL7A_ARCFU] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA and box C/D sRNAs.[1] Publication Abstract from PubMedA recent study has shown that archaeal L7Ae binds to a putative k-turn structure in the 5'-leader of the mRNA of its structural gene to regulate translation. To function as a regulator the RNA should be unstructured in the absence of protein, but it should adopt a k-turn-containing stem-loop on binding L7Ae. Sequence analysis of UTR sequences indicates that their k-turn elements will be unable to fold in the absence of L7Ae, and we have demonstrated this experimentally in solution using FRET for the Archeoglobus fulgidus sequence. We have solved the X-ray crystal structure of the complex of the A. fulgidus RNA bound to its cognate L7Ae protein. The RNA adopts a standard k-turn conformation, that is specifically recognized by the L7Ae protein so stabilizing the stem-loop. In-line probing of the natural-sequence UTR shows that the RNA is unstructured in the absence of L7Ae binding, but folds on binding the protein such that the ribosome binding site is occluded. Thus L7Ae regulates its own translation by switching the conformation of the RNA to alter accessibility. The role of RNA structure in translational regulation by L7Ae protein in archaea.,Huang L, Ashraf S, Lilley DMJ RNA. 2018 Oct 16. pii: rna.068510.118. doi: 10.1261/rna.068510.118. PMID:30327333[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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