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==Water-soluble Chlorophyll Protein (WSCP) from Lepidium virginicum (Mutation L91P) with Chlorophyll-a== | |||
<StructureSection load='6giw' size='340' side='right' caption='[[6giw]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6giw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lepvr Lepvr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GIW FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">WSCP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=59292 LEPVR])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6giw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6giw OCA], [http://pdbe.org/6giw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6giw RCSB], [http://www.ebi.ac.uk/pdbsum/6giw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6giw ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We altered the chlorophyll (Chl) binding sites in various versions of water-soluble chlorophyll protein (WSCP) by amino acid exchanges to alter their preferences for either Chl a or Chl b. WSCP is ideally suited for this mutational analysis since it forms a tetrameric complex with only four identical Chl binding sites. A loop of 4-6 amino acids is responsible for Chl a versus Chl b selectivity. We show that a single amino acid exchange within this loop changes the relative Chl a/b affinities by a factor of 40. We obtained crystal structures of this WSCP variant binding either Chl a or Chl b. The Chl binding sites in these structures were compared with those in the major light-harvesting complex (LHCII) of the photosynthetic apparatus in plants to search for similar structural features involved in Chl a/b binding specificity. | |||
Chlorophyll a/b binding-specificity in water-soluble chlorophyll protein.,Palm DM, Agostini A, Averesch V, Girr P, Werwie M, Takahashi S, Satoh H, Jaenicke E, Paulsen H Nat Plants. 2018 Oct 8. pii: 10.1038/s41477-018-0273-z. doi:, 10.1038/s41477-018-0273-z. PMID:30297830<ref>PMID:30297830</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6giw" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Lepvr]] | |||
[[Category: Agostini, A]] | |||
[[Category: Averesch, V]] | |||
[[Category: Girr, P]] | |||
[[Category: Jaenicke, E]] | |||
[[Category: Palm, D M]] | |||
[[Category: Paulsen, H]] | |||
[[Category: Satoh, H]] | |||
[[Category: Takahashi, S]] | |||
[[Category: Werwie, M]] | |||
[[Category: Chlorophyll]] | |||
[[Category: Chlorophyll carrier]] | |||
[[Category: Lepidium virginicum]] | |||
[[Category: Photooxidation]] | |||
[[Category: Plant]] | |||
[[Category: Plant protein]] | |||
[[Category: Tetramer]] | |||
[[Category: Water-soluble chlorophyll protein]] | |||
Latest revision as of 12:02, 14 November 2018
Water-soluble Chlorophyll Protein (WSCP) from Lepidium virginicum (Mutation L91P) with Chlorophyll-aWater-soluble Chlorophyll Protein (WSCP) from Lepidium virginicum (Mutation L91P) with Chlorophyll-a
Structural highlights
Publication Abstract from PubMedWe altered the chlorophyll (Chl) binding sites in various versions of water-soluble chlorophyll protein (WSCP) by amino acid exchanges to alter their preferences for either Chl a or Chl b. WSCP is ideally suited for this mutational analysis since it forms a tetrameric complex with only four identical Chl binding sites. A loop of 4-6 amino acids is responsible for Chl a versus Chl b selectivity. We show that a single amino acid exchange within this loop changes the relative Chl a/b affinities by a factor of 40. We obtained crystal structures of this WSCP variant binding either Chl a or Chl b. The Chl binding sites in these structures were compared with those in the major light-harvesting complex (LHCII) of the photosynthetic apparatus in plants to search for similar structural features involved in Chl a/b binding specificity. Chlorophyll a/b binding-specificity in water-soluble chlorophyll protein.,Palm DM, Agostini A, Averesch V, Girr P, Werwie M, Takahashi S, Satoh H, Jaenicke E, Paulsen H Nat Plants. 2018 Oct 8. pii: 10.1038/s41477-018-0273-z. doi:, 10.1038/s41477-018-0273-z. PMID:30297830[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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