6gdh: Difference between revisions
New page: '''Unreleased structure''' The entry 6gdh is ON HOLD Authors: Parkinson, G.N., Haider, S., Li, P., Khiali, S., Munnur, D., Ramanathan, A. Description: Holliday Junctions formed from Te... |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Holliday Junctions formed from Telomeric DNA== | |||
<StructureSection load='6gdh' size='340' side='right' caption='[[6gdh]], [[Resolution|resolution]] 2.85Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6gdh]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GDH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GDH FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gdh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gdh OCA], [http://pdbe.org/6gdh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gdh RCSB], [http://www.ebi.ac.uk/pdbsum/6gdh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gdh ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cells have evolved inherent mechanisms, like homologous recombination (HR), to repair damaged DNA. However, repairs at telomeres can lead to genomic instability, often associated with cancer. While most rapidly dividing cells employ telomerase, the others maintain telomere length through HR-dependent alternative lengthening of telomeres (ALT) pathways. Here we describe the crystal structures of Holliday junction intermediates of the HR-dependent ALT mechanism. Using an extended human telomeric repeat, we also report the crystal structure of two Holliday junctions in close proximity, which associate together through strand exchange to form a hemicatenated double Holliday junction. Our combined structural results demonstrate that ACC nucleotides in the C-rich lagging strand (5'-CTAACCCTAA-3') at the telomere repeat sequence constitute a conserved structural feature that constrains crossover geometry and is a preferred site for Holliday junction formation in telomeres. | |||
Holliday Junctions Formed from Human Telomeric DNA.,Haider S, Li P, Khiali S, Munnur D, Ramanathan A, Parkinson GN J Am Chem Soc. 2018 Oct 30. doi: 10.1021/jacs.8b08699. PMID:30376323<ref>PMID:30376323</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6gdh" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Haider, S]] | |||
[[Category: Khiali, S]] | |||
[[Category: Li, P]] | |||
[[Category: Munnur, D]] | [[Category: Munnur, D]] | ||
[[Category: Parkinson, G N]] | |||
[[Category: Parkinson, G | |||
[[Category: Ramanathan, A]] | [[Category: Ramanathan, A]] | ||
[[Category: | [[Category: Acc structural motif]] | ||
[[Category: Alt mechanism]] | |||
[[Category: Holliday junction]] | |||
[[Category: Homologous recombination]] | |||
[[Category: Recombination]] | |||
[[Category: Telomere]] | |||
Latest revision as of 15:17, 7 November 2018
Holliday Junctions formed from Telomeric DNAHolliday Junctions formed from Telomeric DNA
Structural highlights
Publication Abstract from PubMedCells have evolved inherent mechanisms, like homologous recombination (HR), to repair damaged DNA. However, repairs at telomeres can lead to genomic instability, often associated with cancer. While most rapidly dividing cells employ telomerase, the others maintain telomere length through HR-dependent alternative lengthening of telomeres (ALT) pathways. Here we describe the crystal structures of Holliday junction intermediates of the HR-dependent ALT mechanism. Using an extended human telomeric repeat, we also report the crystal structure of two Holliday junctions in close proximity, which associate together through strand exchange to form a hemicatenated double Holliday junction. Our combined structural results demonstrate that ACC nucleotides in the C-rich lagging strand (5'-CTAACCCTAA-3') at the telomere repeat sequence constitute a conserved structural feature that constrains crossover geometry and is a preferred site for Holliday junction formation in telomeres. Holliday Junctions Formed from Human Telomeric DNA.,Haider S, Li P, Khiali S, Munnur D, Ramanathan A, Parkinson GN J Am Chem Soc. 2018 Oct 30. doi: 10.1021/jacs.8b08699. PMID:30376323[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||