6g3e: Difference between revisions
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==Crystal structure of EDDS lyase in complex with formate== | |||
<StructureSection load='6g3e' size='340' side='right' caption='[[6g3e]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6g3e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chesb Chesb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G3E FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Meso_0564 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266779 CHESB])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g3e OCA], [http://pdbe.org/6g3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g3e RCSB], [http://www.ebi.ac.uk/pdbsum/6g3e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g3e ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The natural aminocarboxylic acid product ethylenediamine- N, N'-disuccinic acid [( S, S)-EDDS] is able to form a stable complex with metal ions, making it an attractive biodegradable alternative for the synthetic metal chelator ethylenediaminetetraacetic acid (EDTA), which is currently used on a large scale in numerous applications. Previous studies have demonstrated that biodegradation of ( S, S)-EDDS may be initiated by an EDDS lyase, converting ( S, S)-EDDS via the intermediate N-(2-aminoethyl)aspartic acid (AEAA) into ethylenediamine and two molecules of fumarate. However, current knowledge of this enzyme is limited because of the absence of structural data. Here, we describe the identification and characterization of an EDDS lyase from Chelativorans sp. BNC1, which has a broad substrate scope, accepting various mono- and diamines for addition to fumarate. We report crystal structures of the enzyme in an unliganded state and in complex with formate, succinate, fumarate, AEAA, and ( S, S)-EDDS. The structures reveal a tertiary and quaternary fold that is characteristic of the aspartase/fumarase superfamily and support a mechanism that involves general base-catalyzed, sequential two-step deamination of ( S, S)-EDDS. This work broadens our understanding of mechanistic diversity within the aspartase/fumarase superfamily and will aid in the optimization of EDDS lyase for asymmetric synthesis of valuable (metal-chelating) aminocarboxylic acids. | |||
Structural Basis for the Catalytic Mechanism of Ethylenediamine- N, N'-disuccinic Acid Lyase, a Carbon-Nitrogen Bond-Forming Enzyme with a Broad Substrate Scope.,Poddar H, de Villiers J, Zhang J, Puthan Veetil V, Raj H, Thunnissen AWH, Poelarends GJ Biochemistry. 2018 May 18. doi: 10.1021/acs.biochem.8b00406. PMID:29741885<ref>PMID:29741885</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 6g3e" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Chesb]] | |||
[[Category: Poddar, H]] | [[Category: Poddar, H]] | ||
[[Category: Thunnissem, A | [[Category: Poelarends, G J]] | ||
[[Category: Thunnissem, A M.W H]] | |||
[[Category: Aspartase fumarase superfamily]] | |||
[[Category: C-n lyase]] | |||
[[Category: Edd]] | |||
[[Category: Formate]] | |||
[[Category: Lyase]] | |||
[[Category: Metal chelator]] | |||
[[Category: Tetramer]] | |||