Byron's Bender: Difference between revisions
Eric Martz (talk | contribs) No edit summary |
Eric Martz (talk | contribs) No edit summary |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 2: | Line 2: | ||
[[Image:Byrons-bender-model.gif]] | [[Image:Byrons-bender-model.gif]] | ||
</td></tr><tr><td> | </td></tr><tr><td> | ||
Byron's Bender wire model of the backbone trace of the I domain of CD11a (from [[1lfa]]), an alpha/beta domain of a leukocyte integrin adhesion molecule. Alpha helices are decorated with red pipe cleaners, while beta strands are decorated yellow. Model generously constructed by Tim Herman in 1997. | Byron's Bender wire model of the backbone trace of the I domain of CD11a (from [[1lfa]]), an alpha/beta domain of a leukocyte integrin adhesion molecule. Alpha helices are decorated with red pipe cleaners, while beta strands are decorated yellow. Model generously constructed by Tim Herman in 1997. Photo by [[User:Eric Martz|Eric Martz]]. | ||
</td></tr></table> | </td></tr></table> | ||
Byron's Bender, invented by crystallographer Byron Rubin<ref name="b72">Rubin, Byron; Richardson Jane S. The simple construction of protein alpha-carbon models. Biopolymers. 1972; 11(11):2381-5. [https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.1972.360111116 PDF]</ref><ref name="b85">Rubin, Byron. 1985. Macromolecule backbone models. ''Methods in Enzymology'' '''115''':391-7.</ref>, is a machine designed to bend wire into the backbone trace of a protein model. The machine was manufactured by the Charles Supper Company in Natick Massachusetts<ref name="supper">[http://www.charles-supper.com/en/page/product.cfm?idProduct=33 Supper Protein Wire Model Bender]</ref>, and widely used by crystallographers in the 1970's and 1980's. | Byron's Bender, invented by crystallographer Byron Rubin<ref name="b72">Rubin, Byron; Richardson Jane S. The simple construction of protein alpha-carbon models. Biopolymers. 1972; 11(11):2381-5. [https://onlinelibrary.wiley.com/doi/epdf/10.1002/bip.1972.360111116 PDF]</ref><ref name="b85">Rubin, Byron. 1985. Macromolecule backbone models. ''Methods in Enzymology'' '''115''':391-7.</ref>, is a machine designed to bend wire into the backbone trace of a protein model. The machine was manufactured by the Charles Supper Company in Natick Massachusetts<ref name="supper">[http://www.charles-supper.com/en/page/product.cfm?idProduct=33 Supper Protein Wire Model Bender]</ref>, and widely used by crystallographers in the 1970's and 1980's. | ||
{{Yelspan|Photos of the machine are available elsewhere<ref name="b72" /><ref name="b85" /><ref name="supper" /><ref name="dataphys">[http://dataphys.org/list/byrons-bender/ 1970 - Byron's Bender] by Pierre Dragicevic, 2015.</ref>.}} | |||
Wire is advanced in fixed-length increments, representing the distances between alpha-carbon atoms. A dihedral angle is then set on dials, and a lever is pulled to bend the wire to the specified angle. Often, the emerging wire model collides with the machine. Then, the wire is cut, and later the resulting segments are fastened end to end with small metal sleeves. Each such joint is silver-soldered or glued at the correct angle. | Wire is advanced in fixed-length increments, representing the distances between alpha-carbon atoms. A dihedral angle is then set on dials, and a lever is pulled to bend the wire to the specified angle. Often, the emerging wire model collides with the machine. Then, the wire is cut, and later the resulting segments are fastened end to end with small metal sleeves. Each such joint is silver-soldered or glued at the correct angle. | ||
<table align="left" style="padding | <table align="left" style="padding:8px;background-color:#e8e8e8;" width="420"><tr><td> | ||
[[Image:Byrons bender 600px.jpeg]] | [[Image:Byrons bender 600px.jpeg]] | ||
</td></tr><tr><td> | </td></tr><tr><td> | ||
Byron's bender, alongside wire model created with it. Photo provided by Leonard Banaszak (Univ. Minnesota) to [[User:Bernhard Rupp|Bernhard Rupp]] for use in his 2009 book [https://books.google.com/books?id=gTAWBAAAQBAJ&pg=PA609&lpg=PA609&dq=edgar+meyer+crystallographer&source=bl&ots=x_E9nJyIjZ&sig=Oqm7Mjdaq9otCnrzdiJNAyINd6U&hl=en&sa=X&ved=0ahUKEwiy8ue7zbLaAhVN6Z8KHfrQBJI4ChDoAQgmMAA#v=onepage&q=edgar%20meyer%20crystallographer&f=false Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology]. Used with permission<ref>Permission given in April, 2018 by Bernhard Rupp.</ref>. | Byron's bender, alongside wire model created with it. Photo provided by Leonard Banaszak (Univ. Minnesota) to [[User:Bernhard Rupp|Bernhard Rupp]] for use in his 2009 book [https://books.google.com/books?id=gTAWBAAAQBAJ&pg=PA609&lpg=PA609&dq=edgar+meyer+crystallographer&source=bl&ots=x_E9nJyIjZ&sig=Oqm7Mjdaq9otCnrzdiJNAyINd6U&hl=en&sa=X&ved=0ahUKEwiy8ue7zbLaAhVN6Z8KHfrQBJI4ChDoAQgmMAA#v=onepage&q=edgar%20meyer%20crystallographer&f=false Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology]. Used with permission<ref>Permission given in April, 2018 by Bernhard Rupp.</ref>. Below: Detail. | ||
</td></tr><tr><td> | |||
[[Image:Byrons bender detail.jpg|600px]] | |||
</td></tr></table> | </td></tr></table> | ||