6exb: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6exb is ON HOLD  until Paper Publication
+==Crystal structure of DotM cytoplasmic domain (residues 153-380), native form==
+<StructureSection load='6exb' size='340' side='right' caption='[[6exb]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6exb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EXB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EXB FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">icmP, lpg0445 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6exb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6exb OCA], [http://pdbe.org/6exb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6exb RCSB], [http://www.ebi.ac.uk/pdbsum/6exb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6exb ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Legionella pneumophila, a causative agent of pneumonia, utilizes the Type 4B secretion (T4BS) system to translocate over 300 effectors into the host cell during infection. T4BS systems are encoded by a large gene cluster termed dot/icm, three components of which, DotL, DotM, and DotN, form the "coupling complex", which serves as a platform for recruitment of effector proteins. One class of effectors includes proteins containing Glu-rich/E-block sequences at their C terminus. However, the protein or region of the coupling complex mediating recruitment of such effectors is unknown. Here we present the crystal structure of DotM. This all alpha-helical structure exhibits patches of positively charged residues. We show that these regions form binding sites for acidic Glu-rich peptides and that mutants targeting these patches are defective in vivo in the translocation of acidic Glu-rich motif-containing effectors. We conclude that DotM forms the interacting surface for recruitment of acidic Glu-rich motif-containing Legionella effectors.
-Authors:
+Legionella DotM structure reveals a role in effector recruiting to the Type 4B secretion system.,Meir A, Chetrit D, Liu L, Roy CR, Waksman G Nat Commun. 2018 Feb 6;9(1):507. doi: 10.1038/s41467-017-02578-x. PMID:29410427<ref>PMID:29410427</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6exb" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Legph]]
+[[Category: Meir, A]]
+[[Category: Waksman, G]]
+[[Category: Coupling complex]]
+[[Category: Legionella pneumophila]]
+[[Category: Membrane protein]]
+[[Category: Protein binding]]
+[[Category: Secretion system]]
+[[Category: Type 4 secretion system]]