6em0: Difference between revisions
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<StructureSection load='6em0' size='340' side='right' caption='[[6em0]], [[Resolution|resolution]] 2.78Å' scene=''> | <StructureSection load='6em0' size='340' side='right' caption='[[6em0]], [[Resolution|resolution]] 2.78Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6em0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EM0 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6em0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"pseudomonas_azelaica"_janota-bassalik_et_al._1971 "pseudomonas azelaica" janota-bassalik et al. 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EM0 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hbpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46680 "Pseudomonas azelaica" Janota-Bassalik et al. 1971])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6em0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6em0 OCA], [http://pdbe.org/6em0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6em0 RCSB], [http://www.ebi.ac.uk/pdbsum/6em0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6em0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6em0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6em0 OCA], [http://pdbe.org/6em0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6em0 RCSB], [http://www.ebi.ac.uk/pdbsum/6em0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6em0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Pseudomonas azelaica janota-bassalik et al. 1971]] | |||
[[Category: Benhar, Y Pazy]] | [[Category: Benhar, Y Pazy]] | ||
[[Category: Bregman-Cohen, A]] | [[Category: Bregman-Cohen, A]] | ||
Latest revision as of 11:28, 17 January 2018
Crystal Structure of 2-hydroxybiphenyl 3-monooxygenase M321A from Pseudomonas azelaicaCrystal Structure of 2-hydroxybiphenyl 3-monooxygenase M321A from Pseudomonas azelaica
Structural highlights
Publication Abstract from PubMed2-Hydroxybiphenyl 3-monooxygenase (HbpA) is a flavin-containing NADH-dependent aromatic hydroxylase that oxidizes a broad range of 2-substituted phenols. In order to modulate its activity and selectivity, several residues in the active site pocket were investigated by saturation mutagenesis. Variant M321A demonstrated altered regioselectivity by oxidizing for the first time 3-hydroxybiphenyl, thus enabling the production of a new antioxidant, 3,4-dihydroxybiphenyl, with similar ferric reducing capacity as the well-studied piceatannol. The crystal structure of M321A was determined (2.78 A) and molecular docking of the 3-substituted phenol provided a rational explanation for the altered regioselectivity. Furthermore, HbpA was found to possess pro-S enantioselectivity towards the production of several chiral sulfoxides, while variant M321F exhibited improved enantioselectivity. Based on biochemical characterization of several mutants, it was suggested that Trp97 stabilizes the substrate in the active site, Met223 is involved in NADH entrance or binding to the active site, while Pro320 may facilitate FAD movement. Altering 2-hydroxybiphenyl 3-monooxygenase regioselectivity by protein engineering for the production of a new antioxidant.,Bregman-Cohen A, Deri B, Maimon S, Pazy Y, Fishman A Chembiochem. 2018 Jan 3. doi: 10.1002/cbic.201700648. PMID:29297973[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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