V-ATPase: Difference between revisions

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Alexander Berchansky, Gydo van Zundert, Michal Harel

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 ==Introduction==
-Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes.
+Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes. V-ATPases are structurally and mechanically related to F- and A-ATPases.<ref>PMID:15473999</ref>
 ==V-ATPase components==
-The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units and which transports the protons.<ref>PMID:20450191</ref>
+The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units (a, d, e, c, c' and c") and which acts as a proton translocator from the cytoplasm to the lumen.<ref>PMID:20450191</ref>
-==Mechanism of rotation==
+ATP hydrolysis occurs at catalytic sites located at the interface of the A and B subunits.
-==V-ATPase structures==
+===V1-domain===
+<StructureSection load='3a5c' size='250' side='right' caption='V1-domain of V-ATPase of Thermus Thermophilus (PDB entry [[3j0j]])' scene='V-ATPase/V1-part/1'>
+The <scene name='V-ATPase/V1-part/1'>V1-domain</scene> consist of the A3B3 complex where the hydrolysis of ATP occurs (<scene name='V-ATPase/V1-part/3'>A is blue, B is red</scene>), subunit C (<scene name='V-ATPase/V1-part/8'>green</scene>), the D-subunit which is the central stalk (<scene name='V-ATPase/V1-part/2'>purple</scene>), subunit E (<scene name='V-ATPase/V1-part/6'>orange</scene>), subunit F (<scene name='V-ATPase/V1-part/4'>yellow</scene>)and subunit G (<scene name='V-ATPase/V1-part/7'>dark green</scene>).
+</StructureSection>
-===PDB===
+===V0-domain===
-====V1 complex====
-[[3j0j]]: Fitted structure of Thermus Thermophilus in a 9.7&Aring; resolution cryo-EM map.
-[[3a5c]]
-[[3a5d]]
-====A3B3 complex====
-[[3gqb]]
-====Subunit C====
-[[1r5z]]
-[[1u7l]]
-[[1v9m]]
-====Subunit E====
-[[2kz9]]
-[[3k5b]]
-[[3v6i]]
-====Subunit F====
-[[2d00]]
-====Subunit G====
-[[2kwy]]
-[[2k88]]
-====Subunit H====
-[[1ho8]]
-====Vo complex====
-[[3aou]]
-[[2db4]]
-[[2bl2]]
-[[2cyd]]
-====Subunit a====
-[[2rpw]]
-[[2nvj]]
+==Mechanism of rotation==
+==3D V-ATPase structures==
+See [[ATPase]]
 ===EMDB===