OspA L03 Group2: Difference between revisions

These spirochetes however do not enter the host through salivary gland secretions into the blood stream. The host becomes infected because the tick's blood meal enters the gut where the spirochetes are located where some of the blood that returns to the bitten area is infected with borrelia. This results in the transmission into the host's blood stream <ref name=Onrust>PMID: 10730551</ref>). Once the bacteria is in the blood stream, the immune system may, or may not destroy this [http://en.wikipedia.org/wiki/Pathogen pathogen], depending on the presence of a [http://en.wikipedia.org/wiki/Vaccine vaccine]. The type of proteins on the surface of the borrelia detected by the host's immune system determines the fate of the survival of this disease. One of the main outer surface proteins, OspA, plays a major role in the ability of the immune system to destroy these [http://en.wikipedia.org/wiki/Antigen antigens] <ref name=Ding >PMID: 11183781</ref>.

<Structure load='1FJ1' size='400' align='left' caption='OspA (magenta, cyan) complex with antibody light chain (grey, pink) and heavy chain (gree, yellow) (PDB code [[1fj1]])' scene='OspA_L03_Group2/Default/1' />

The following is the fit mechanism where the conformations recognize LA-2 and shifts to optimize the complementary antigen combing site <ref name=Ding >PMID: 11183781</ref>. There were 49 residues from the “three loops” involved that significantly affected by LA-2 binding, through findings from NMR and crystallization <ref name=Ding >PMID: 11183781</ref>.  Residues 207 and 227 were excluded from analysis because of the peak overlap <ref name=Ding >PMID: 11183781</ref>. The portion of the protein chain detected through 15N-HSQC NMR that were affected by the binding of LA2 <ref name=Ding >PMID: 11183781</ref> was highlighted.<scene name='OspA_L03_Group2/Residues_203-220_loop1/2'>Residues 203 to 220 in "loop 1"</scene>” were represented by purple, <scene name='OspA_L03_Group2/Residues_224-233_loop2/2'>residues 224-233 in loop 2</scene> were colored green and <scene name='OspA_L03_Group2/Residues_246-257_loop3/3'>residues 247-257 in "loop 3"</scene>” were colored aqua blue The cool coloring of the residues shows the location of LA-2’s direct contact on the “3 loops”. Primary colors were used to represent <scene name='OspA_L03_Group2/Ala_208/4'>Ala-208</scene> as orange and <scene name='OspA_L03_Group2/Ala_215/4'>Ala-215</scene> as yellow in spacefills for the primary or initial identification of the LA-2 epitope on the beta-strands. The coloration of resides are all at one end, C-terminal of the isolated OspA molecule showing the side where LA-2 binds. The rest of the model were beta-sheets that were left yellow, as the neutral color, and the only alpha-helix was colored pink, to show the general overall structure of 21 anti-parrallel beta-strands to 1 alpha-helix <ref name=Ding >PMID: 11183781</ref>.