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[[Image:2cn1.gif|left|200px]]<br /><applet load="2cn1" size="350" color="white" frame="true" align="right" spinBox="true"  
==CRYSTAL STRUCTURE OF HUMAN CYTOSOLIC 5'-NUCLEOTIDASE III (NT5C3)(CASP TARGET)==
caption="2cn1, resolution 2.67&Aring;" />
<StructureSection load='2cn1' size='340' side='right' caption='[[2cn1]], [[Resolution|resolution]] 2.67&Aring;' scene=''>
'''CRYSTAL STRUCTURE OF HUMAN CYTOSOLIC 5'-NUCLEOTIDASE III (NT5C3)(CASP TARGET)'''<br />
== Structural highlights ==
<table><tr><td colspan='2'>[[2cn1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CN1 FirstGlance]. <br>
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cn1 OCA], [http://pdbe.org/2cn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cn1 RCSB], [http://www.ebi.ac.uk/pdbsum/2cn1 PDBsum]</span></td></tr>
</table>
== Disease ==
[[http://www.uniprot.org/uniprot/5NT3_HUMAN 5NT3_HUMAN]] Defects in NT5C3 are the cause of P5N deficiency (P5ND) [MIM:[http://omim.org/entry/266120 266120]]; also called hemolytic anemia due to P5N deficiency or hemolytic anemia due to UMPH1 deficiency. P5ND is an autosomal recessive condition causing hemolytic anemia characterized by marked basophilic stipplig and the accumulation of high concentrations of pyrimidine nucleotides within the erythrocyte. It is implicated in the anemia of lead poisoning and is possibly associated with learning difficulties.
== Function ==
[[http://www.uniprot.org/uniprot/5NT3_HUMAN 5NT3_HUMAN]] Can act both as nucleotidase and as phosphotransferase.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/2cn1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cn1 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cytosolic 5'-nucleotidase II catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates and regulates the IMP and GMP pools within the cell. It possesses phosphotransferase activity and thereby also catalyzes the reverse reaction. Both reactions are allosterically activated by adenine-based nucleotides and 2,3-bisphosphoglycerate. We have solved structures of cytosolic 5'-nucleotidase II as native protein (2.2 Angstrom) and in complex with adenosine (1.5 Angstrom) and beryllium trifluoride (2.15 Angstrom) The tetrameric enzyme is structurally similar to enzymes of the haloacid dehalogenase (HAD) superfamily, including mitochondrial 5'(3')-deoxyribonucleotidase and cytosolic 5'-nucleotidase III but possesses additional regulatory regions that contain two allosteric effector sites. At effector site 1 located near a subunit interface we modeled diadenosine tetraphosphate with one adenosine moiety in each subunit. This efficiently glues the tetramer subunits together in pairs. The model shows why diadenosine tetraphosphate but not diadenosine triphosphate activates the enzyme and supports a role for cN-II during apoptosis when the level of diadenosine tetraphosphate increases. We have also modeled 2,3-bisphosphoglycerate in effector site 1 using one phosphate site from each subunit. By comparing the structure of cytosolic 5'-nucleotidase II with that of mitochondrial 5'(3')-deoxyribonucleotidase in complex with dGMP, we identified residues involved in substrate recognition.


==Disease==
Crystal structure of human cytosolic 5'-nucleotidase II: insights into allosteric regulation and substrate recognition.,Wallden K, Stenmark P, Nyman T, Flodin S, Graslund S, Loppnau P, Bianchi V, Nordlund P J Biol Chem. 2007 Jun 15;282(24):17828-36. Epub 2007 Apr 3. PMID:17405878<ref>PMID:17405878</ref>
Known diseases associated with this structure: Anemia, hemolytic, due to UMPH1 deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=606224 606224]]


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2CN1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CN1 OCA].
</div>
<div class="pdbe-citations 2cn1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: 5'-nucleotidase]]
[[Category: 5'-nucleotidase]]
[[Category: Homo sapiens]]
[[Category: Human]]
[[Category: Single protein]]
[[Category: Arrowsmith, C]]
[[Category: Arrowsmith, C.]]
[[Category: Berg, S Van Den]]
[[Category: Berg, S Van Den.]]
[[Category: Berglund, H]]
[[Category: Berglund, H.]]
[[Category: Collins, R]]
[[Category: Collins, R.]]
[[Category: Edwards, A]]
[[Category: Edwards, A.]]
[[Category: Ehn, M]]
[[Category: Ehn, M.]]
[[Category: Flodin, S]]
[[Category: Flodin, S.]]
[[Category: Flores, A]]
[[Category: Flores, A.]]
[[Category: Graslund, S]]
[[Category: Graslund, S.]]
[[Category: Hallberg, B M]]
[[Category: Hallberg, B M.]]
[[Category: Hammarstrom, M]]
[[Category: Hammarstrom, M.]]
[[Category: Hogbom, M]]
[[Category: Hogbom, M.]]
[[Category: Kotenyova, T]]
[[Category: Kotenyova, T.]]
[[Category: Magnusdottir, A]]
[[Category: Magnusdottir, A.]]
[[Category: Nilsson-Ehle, P]]
[[Category: Nilsson-Ehle, P.]]
[[Category: Nordlund, P]]
[[Category: Nordlund, P.]]
[[Category: Nyman, T]]
[[Category: Nyman, T.]]
[[Category: Ogg, D]]
[[Category: Ogg, D.]]
[[Category: Persson, C]]
[[Category: Persson, C.]]
[[Category: Sagemark, J]]
[[Category: Sagemark, J.]]
[[Category: Schiavone, L Holmberg]]
[[Category: Schiavone, L Holmberg.]]
[[Category: Stenmark, P]]
[[Category: Stenmark, P.]]
[[Category: Sundstrom, M]]
[[Category: Sundstrom, M.]]
[[Category: Thorsell, A G]]
[[Category: Thorsell, A G.]]
[[Category: Uppenberg, J]]
[[Category: Uppenberg, J.]]
[[Category: Wallden, K]]
[[Category: Wallden, K.]]
[[Category: Weigelt, J]]
[[Category: Weigelt, J.]]
[[Category: Welin, M]]
[[Category: Welin, M.]]
[[Category: 5-prime]]
[[Category: 5-prime]]
[[Category: alternative splicing]]
[[Category: Hydrolase]]
[[Category: cytosolic iii]]
[[Category: Nucleotidase]]
[[Category: disease mutation]]
[[Category: Nucleotide-binding]]
[[Category: hydrolase]]
[[Category: Umph1]]
[[Category: nucleotidase]]
[[Category: nucleotide metabolism]]
[[Category: nucleotide-binding]]
[[Category: p5n1]]
[[Category: pyrimidine 5-prime nucleotidase deficiency]]
[[Category: pyrimidine 5-prime- nucleotidase 1]]
[[Category: umph1]]
[[Category: uridine 5-prime monophosphate hydrolase 1]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:50:20 2008''

Latest revision as of 08:28, 10 February 2016

CRYSTAL STRUCTURE OF HUMAN CYTOSOLIC 5'-NUCLEOTIDASE III (NT5C3)(CASP TARGET)CRYSTAL STRUCTURE OF HUMAN CYTOSOLIC 5'-NUCLEOTIDASE III (NT5C3)(CASP TARGET)

Structural highlights

2cn1 is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Activity:5'-nucleotidase, with EC number 3.1.3.5
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum

Disease

[5NT3_HUMAN] Defects in NT5C3 are the cause of P5N deficiency (P5ND) [MIM:266120]; also called hemolytic anemia due to P5N deficiency or hemolytic anemia due to UMPH1 deficiency. P5ND is an autosomal recessive condition causing hemolytic anemia characterized by marked basophilic stipplig and the accumulation of high concentrations of pyrimidine nucleotides within the erythrocyte. It is implicated in the anemia of lead poisoning and is possibly associated with learning difficulties.

Function

[5NT3_HUMAN] Can act both as nucleotidase and as phosphotransferase.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cytosolic 5'-nucleotidase II catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates and regulates the IMP and GMP pools within the cell. It possesses phosphotransferase activity and thereby also catalyzes the reverse reaction. Both reactions are allosterically activated by adenine-based nucleotides and 2,3-bisphosphoglycerate. We have solved structures of cytosolic 5'-nucleotidase II as native protein (2.2 Angstrom) and in complex with adenosine (1.5 Angstrom) and beryllium trifluoride (2.15 Angstrom) The tetrameric enzyme is structurally similar to enzymes of the haloacid dehalogenase (HAD) superfamily, including mitochondrial 5'(3')-deoxyribonucleotidase and cytosolic 5'-nucleotidase III but possesses additional regulatory regions that contain two allosteric effector sites. At effector site 1 located near a subunit interface we modeled diadenosine tetraphosphate with one adenosine moiety in each subunit. This efficiently glues the tetramer subunits together in pairs. The model shows why diadenosine tetraphosphate but not diadenosine triphosphate activates the enzyme and supports a role for cN-II during apoptosis when the level of diadenosine tetraphosphate increases. We have also modeled 2,3-bisphosphoglycerate in effector site 1 using one phosphate site from each subunit. By comparing the structure of cytosolic 5'-nucleotidase II with that of mitochondrial 5'(3')-deoxyribonucleotidase in complex with dGMP, we identified residues involved in substrate recognition.

Crystal structure of human cytosolic 5'-nucleotidase II: insights into allosteric regulation and substrate recognition.,Wallden K, Stenmark P, Nyman T, Flodin S, Graslund S, Loppnau P, Bianchi V, Nordlund P J Biol Chem. 2007 Jun 15;282(24):17828-36. Epub 2007 Apr 3. PMID:17405878[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Wallden K, Stenmark P, Nyman T, Flodin S, Graslund S, Loppnau P, Bianchi V, Nordlund P. Crystal structure of human cytosolic 5'-nucleotidase II: insights into allosteric regulation and substrate recognition. J Biol Chem. 2007 Jun 15;282(24):17828-36. Epub 2007 Apr 3. PMID:17405878 doi:http://dx.doi.org/10.1074/jbc.M700917200

2cn1, resolution 2.67Å

Drag the structure with the mouse to rotate

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