User:Jessica Bun/Sandbox1: Difference between revisions

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== Function ==

~~The current biochemical function~~ is ~~unknown~~, ~~but it~~ has ~~been proposed~~ to ~~play~~ a ~~role in magnesium uptake~~.

This domain of MgtC, in contrast, is highly variable in comparison to several orthologues, as presented by Yang et al. However, through a sequence alignment of five known functional MgtC orthologues from pathogens that survive inside macrophages (''M. tuberculosis, B. melitensis, B. cenocepacia, Y. pestis,'' and ''S. Typhimurium''), seven strictly conserved residues were found to be scattered along the whole sequence of the relatively hydrophilic and soluble C-terminal domain.

A large hydrophobic core has conserved residues <scene name='69/698113/Conserved_core_residues/3'>Cysteine-155, Arginine-164, Glutamine-160, and Alanine-195</scene>.

[[Image:Final_Final_Core_Logo.PNG |625× 116px|thumb|left|Four strictly conserved residues of five known functional MgtC orthologs of the soluble C-terminal domain.]]

The opposite side of the protein has a small cluster of conserved residues <scene name='69/698113/Conserved_surface_residues/1'>Tyrosine-149, Glutamine-208, and Tryptophan-225</scene>.

[[Image:Final_Surface_Web_Logo.PNG |625× 121px|thumb|left|Four strictly conserved residues of five known functional MgtC orthologs of the soluble C-terminal domain.]]

[[Image:Entire_Web_Logo.PNG |617 × 125 px|thumb|left|Seven strictly conserved residues of five known functional MgtC orthologs of the soluble C-terminal domain.]]

In the

Additionally, there is a crystal structure available for this domain. When comparing the crystal structure of the C-terminal domain to other protein structures, there are striking similarities between this domain and a class of proteins known as ACT domains.

===Mechanism===

@@ Line 7: / Line 7: @@
 == Function ==
-The current biochemical function is unknown, but it has been proposed to play a role in magnesium uptake.
+This domain of MgtC, in contrast, is highly variable in comparison to several orthologues, as presented by Yang et al. However, through a sequence alignment of five known functional MgtC orthologues from pathogens that survive inside macrophages (''M. tuberculosis, B. melitensis, B. cenocepacia, Y. pestis,'' and ''S. Typhimurium''), seven strictly conserved residues were found to be scattered along the whole sequence of the relatively hydrophilic and soluble C-terminal domain.
+A large hydrophobic core has conserved residues <scene name='69/698113/Conserved_core_residues/3'>Cysteine-155, Arginine-164, Glutamine-160, and Alanine-195</scene>.
+[[Image:Final_Final_Core_Logo.PNG |625× 116px|thumb|left|Four strictly conserved residues of five known functional MgtC orthologs of the soluble C-terminal domain.]]
+The opposite side of the protein has a small cluster of conserved residues <scene name='69/698113/Conserved_surface_residues/1'>Tyrosine-149, Glutamine-208, and Tryptophan-225</scene>.
+[[Image:Final_Surface_Web_Logo.PNG |625× 121px|thumb|left|Four strictly conserved residues of five known functional MgtC orthologs of the soluble C-terminal domain.]]
+[[Image:Entire_Web_Logo.PNG |617 × 125 px|thumb|left|Seven strictly conserved residues of five known functional MgtC orthologs of the soluble C-terminal domain.]]
+In the
+Additionally, there is a crystal structure available for this domain.  When comparing the crystal structure of the C-terminal domain to other protein structures, there are striking similarities between this domain and a class of proteins known as ACT domains.
 ===Mechanism===