Molecular Playground/T7 RNAP Conformations: Difference between revisions
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= Conformational Changes on T7 RNA Polymerase = | = Conformational Changes on T7 RNA Polymerase = | ||
[http://en.wikipedia.org/wiki/Transcription_(genetics) Transcription] is a fundamental part of genetic regulation. The RNA polymerases that accomplish this function vary in structure, size and complexity, but must all carry out the same basic functions ([See[http://en.wikipedia.org/wiki/RNA_polymerase]''RNA polymerases''). The correct transcription of DNA to RNA depends of several factors and the complexity increases with the complexity of the organism. This makes the study of the transcriptional process complicated. The RNA polymerase of the [http://ecoliwiki.net/colipedia/index.php/Phage_T7 bacteriophage T7], is the perfect model for studying the transcription process given that T7 RNA polymerase is a single unit enzyme that processes RNA with the same effectivity as the polymerase from higher organisms. Nevertheless, there is plenty to learn from the transcription mechanism, such as the "abortive cycle" process that takes place during the <scene name='User:Luis_E_Ramirez-Tapia/Sandbox_3/Initiation/2'>INITIATION</scene> phase (Figure 1) remains poorly understood. | [http://en.wikipedia.org/wiki/Transcription_(genetics) Transcription] is a fundamental part of genetic regulation. The '''RNA polymerases''' that accomplish this function vary in structure, size and complexity, but must all carry out the same basic functions ([See[http://en.wikipedia.org/wiki/RNA_polymerase]''RNA polymerases''). The correct transcription of DNA to RNA depends of several factors and the complexity increases with the complexity of the organism. This makes the study of the transcriptional process complicated. The RNA polymerase of the [http://ecoliwiki.net/colipedia/index.php/Phage_T7 bacteriophage T7], is the perfect model for studying the transcription process given that T7 RNA polymerase is a single unit enzyme that processes RNA with the same effectivity as the polymerase from higher organisms. Nevertheless, there is plenty to learn from the transcription mechanism, such as the "abortive cycle" process that takes place during the <scene name='User:Luis_E_Ramirez-Tapia/Sandbox_3/Initiation/2'>INITIATION</scene> phase (Figure 1) remains poorly understood. | ||
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[[Image:Abortivecycling.png|thumb|400px|left|<b> Figure 1. Abortive Cycle during transcription initiation</b>]] | [[Image:Abortivecycling.png|thumb|400px|left|<b> Figure 1. Abortive Cycle during transcription initiation</b>]] | ||