Rtp and Tus DNA Binding: Difference between revisions

Latest revision as of 13:16, 4 April 2013

Replication Termination ProteinsReplication Termination Proteins

Rtp and Tus These polar fork bocking sites have been found in yeast, pea, frog and human genomes.

RTPRTP

RTP is a DNA binding protein from Bacillus Subtilis that uses a helix-loop-helix binding motif. In solution it shows a symmetric structure typical of the winged helix loop helix family, with an unstructured end, first alpha helix , unstructured loop that is equivalent to the first beta sheet , helix loop helix structure (-), 2 beta sheets with a connecting loop that makes up the 'wing' structure and an additional long alpha helix involved in dimerisation .

Assymmetry of RTP in DNA-binding

: Contacts upstream with rtp dimer bound to A-site

: Contacts with phosphate backbone of downstream DNA

Residues binding to DNA

3D structures of replication termination protein3D structures of replication termination protein

Replication Termination Protein

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Craig Mooney, Michal Harel

@@ Line 6: / Line 6: @@
 ==RTP==
-RTP is a DNA binding protein from Bacillus Subtilis that uses a helix-loop-helix binding motif. In solution it shows a symmetric structure typical of the winged helix loop helix family, with an unstructured N-terminus end, first alpha helix (a1), unstructured loop that is equivalent to the first beta sheet (B1), helix loop helix structure (<scene name='Rtp_and_Tus_DNA_Binding/Alpha2/1'>a2</scene>-<scene name='Rtp_and_Tus_DNA_Binding/Alpha3/1'>a3</scene> a3), 2 beta sheets with a connecting loop that makes up the 'wing' structure <scene name='Rtp_and_Tus_DNA_Binding/Wing/1'>(B2 - B3)</scene> and an additional long alpha helix involved in dimerisation <scene name='Rtp_and_Tus_DNA_Binding/Alpha4/1'>(a4)</scene>.
+RTP is a DNA binding protein from Bacillus Subtilis that uses a helix-loop-helix binding motif. In solution it shows a symmetric structure typical of the winged helix loop helix family, with an unstructured <scene name='Rtp_and_Tus_DNA_Binding/Nterm/1'>N-terminus</scene> end, first alpha helix <scene name='Rtp_and_Tus_DNA_Binding/Alpha1/1'>(a1)</scene>, unstructured loop that is equivalent to the first beta sheet <scene name='Rtp_and_Tus_DNA_Binding/Beta1/1'>(B1)</scene>, helix loop helix structure (<scene name='Rtp_and_Tus_DNA_Binding/Alpha2/1'>a2</scene>-<scene name='Rtp_and_Tus_DNA_Binding/Alpha3/1'>a3</scene>), 2 beta sheets with a connecting loop that makes up the 'wing' structure <scene name='Rtp_and_Tus_DNA_Binding/Wing/1'>(B2 - B3)</scene> and an additional long alpha helix involved in dimerisation <scene name='Rtp_and_Tus_DNA_Binding/Alpha4/1'>(a4)</scene>.
-The RTP binding site consists of a 30bp sequence consisting of two inperfect 16 bp repeats, the A site and the B site.
+Assymmetry of RTP in DNA-binding
-<scene name='Rtp_and_Tus_DNA_Binding/Overview/1'>Overview</scene>
+<scene name='Rtp_and_Tus_DNA_Binding/Wingup/1'>Wing-up</scene>: Contacts upstream with rtp dimer bound to A-site
+<scene name='Rtp_and_Tus_DNA_Binding/Wingdown/1'>Wing-down</scene>: Contacts with phosphate backbone of downstream DNA
-<Structure load='Lol1.pdb' size='400' color='white'/>
+Residues binding to DNA
+<Structure load='Lol1.pdb' size='400' caption= color='white'/>
+==3D structures of replication termination protein==
+[[Replication Termination Protein]]