Sandbox Reserved 711: Difference between revisions
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The endo-polygalacturonase II (Polygalacturonase EC 3.2.1.15) of the fungus ''Aspergillus Niger'' is an enzyme which is involved in the rotting process. It specifically degrade polygalacturonate, a major carbohydrate constituent of plant cell wall pectin, by hydrolysis of the glycosidic bonds that link galacturonic acid residues. | The endo-polygalacturonase II (Polygalacturonase EC 3.2.1.15) of the fungus ''Aspergillus Niger'' is an enzyme which is involved in the rotting process. It specifically degrade polygalacturonate, a major carbohydrate constituent of plant cell wall pectin, by hydrolysis of the glycosidic bonds that link galacturonic acid residues. | ||
Polygalacturonase belongs to the Glycoside hydrolysases 28 (GH28) according to the sequence-based classification of glycoside hydrolysases. The enzyme of the GH28 family achieves the hydrolysis with an inverting molecular mechanism. | Polygalacturonase belongs to the Glycoside hydrolysases 28 (GH28) according to the sequence-based classification of glycoside hydrolysases. The enzyme of the GH28 family achieves the hydrolysis with an inverting molecular mechanism.<ref>http://www.cazypedia.org/index.php/Inverting#Mechanistic_classification</ref> | ||
== Glycoside hydrolases and pectinases == | == Glycoside hydrolases and pectinases == | ||
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== Description of the structure == | == Description of the structure == | ||
{{STRUCTURE_1czf| PDB=1czf | SCENE= }} | |||
The endopolygalacturonase II from Aspergillus niger has a beta-helix structure<ref>http://www.ncbi.nlm.nih.gov/pubmed/10521427</ref>: the chain folds into 10 turns, shaping the faces of a right-handed helix consisting in 4 parallel beta-sheets - PB1, PB2a, PB2b and PB3 - separated by loops. The helix is closed at its N-terminal end by a <scene name='Sandbox_Reserved_711/N_terminal_alpha_helix/1'>small alpha-helix</scene>. | The endopolygalacturonase II from Aspergillus niger has a beta-helix structure<ref>http://www.ncbi.nlm.nih.gov/pubmed/10521427</ref>: the chain folds into 10 turns, shaping the faces of a right-handed helix consisting in 4 parallel beta-sheets - PB1, PB2a, PB2b and PB3 - separated by loops. The helix is closed at its N-terminal end by a <scene name='Sandbox_Reserved_711/N_terminal_alpha_helix/1'>small alpha-helix</scene>. | ||