Sandbox Reserved 718: Difference between revisions
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<big>''' | <big>'''LIN28: A RNA binding protein'''</big> | ||
{{STRUCTURE_2cqf| PDB=2cqf | SCENE=Sandbox_Reserved_718/Lin28a/1 }} | {{STRUCTURE_2cqf| PDB=2cqf | SCENE=Sandbox_Reserved_718/Lin28a/1 }} | ||
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== '''Structure''' == | == '''Structure''' == | ||
As mentioned | As mentioned above, Lin28 does consist of two domains: a cold shock domain and a <scene name='Sandbox_Reserved_718/Zfd/1'>pair of CCHC zinc fingers</scene>. | ||
Both are RNA binding domains. The cold-shock domain (CSD) at the N-terminal part of Lin28A, residues 37-112<ref>Loughlin, F. E., Gebert, L. F. R., Towbin, H., Brunschweiger, A., Hall, J. and Allain, F. H.-T.: ''Structural basis of pre-let-7 miRNA recognition by the zinc knuckles of pluripotency factor Lin28'' In: Nature Structural & Molecular Biology 19, S. 84-89, Dezember 11, 2012</ref>, interacts with a loop of the RNA, which has a GNGAY motif. | Both are RNA binding domains. The cold-shock domain (CSD) at the N-terminal part of Lin28A, residues 37-112<ref>Loughlin, F. E., Gebert, L. F. R., Towbin, H., Brunschweiger, A., Hall, J. and Allain, F. H.-T.: ''Structural basis of pre-let-7 miRNA recognition by the zinc knuckles of pluripotency factor Lin28'' In: Nature Structural & Molecular Biology 19, S. 84-89, Dezember 11, 2012</ref>, interacts with a loop of the RNA, which has a GNGAY motif. | ||
The other domain, residues 137-176<ref>Loughlin, F. E., Gebert, L. F. R., Towbin, H., Brunschweiger, A., Hall, J. and Allain, F. H.-T.: ''Structural basis of pre-let-7 miRNA recognition by the zinc knuckles of pluripotency factor Lin28'' In: Nature Structural & Molecular Biology 19, S. 84-89, Dezember 11, 2012</ref>, has the binding element AAGNNG (most frequent sequence: AAGGAG) which can be found downstream of the GNGAY motif. The first G builds a <scene name='Sandbox_Reserved_718/Hydrogenbond/2'>hydrogen bond</scene> with <scene name='Sandbox_Reserved_718/Lys160/2'>LYS160</scene> in the zinc finger motif. <ref>Jun Cho, Hyeshik Chang, S. Chul Kwon, Baekgyu Kim, Yoosik Kim, Junho Choe, Minju Ha, Yoon Ki Kim, and V. Narry Kim: ''Lin28A Is a Suppressor of ER-Associated Translation in Embryonic Stem Cells.'' In: Cell 151, S.765–777, November 9, 2012</ref> | The other domain, residues 137-176<ref>Loughlin, F. E., Gebert, L. F. R., Towbin, H., Brunschweiger, A., Hall, J. and Allain, F. H.-T.: ''Structural basis of pre-let-7 miRNA recognition by the zinc knuckles of pluripotency factor Lin28'' In: Nature Structural & Molecular Biology 19, S. 84-89, Dezember 11, 2012</ref>, has the binding element AAGNNG (most frequent sequence: AAGGAG) which can be found downstream of the GNGAY motif. The first G builds a <scene name='Sandbox_Reserved_718/Hydrogenbond/2'>hydrogen bond</scene> with <scene name='Sandbox_Reserved_718/Lys160/2'>LYS160</scene> in the zinc finger motif. <ref>Jun Cho, Hyeshik Chang, S. Chul Kwon, Baekgyu Kim, Yoosik Kim, Junho Choe, Minju Ha, Yoon Ki Kim, and V. Narry Kim: ''Lin28A Is a Suppressor of ER-Associated Translation in Embryonic Stem Cells.'' In: Cell 151, S.765–777, November 9, 2012</ref> | ||
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Katrin Frohnmüller, Teresa Wiese | Katrin Frohnmüller, Teresa Wiese | ||