|
|
| (8 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| [[Image:1uvw.gif|left|200px]] | | #REDIRECT [[3zdw]] This PDB entry is obsolete and replaced by 3zdw |
| | |
| {{Structure
| |
| |PDB= 1uvw |SIZE=350|CAPTION= <scene name='initialview01'>1uvw</scene>, resolution 2.45Å
| |
| |SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>
| |
| |LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=EBS:3-ETHYL-2-[(2Z)-2-(3-ETHYL-6-SULFO-1,3-BENZOTHIAZOL-2(3H)-YLIDENE)HYDRAZINO]-6-SULFO-3H-1,3-BENZOTHIAZOL-1-IUM'>EBS</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
| |
| |ACTIVITY=
| |
| |GENE=
| |
| }}
| |
| | |
| '''BACILLUS SUBTILIS COTA LACCASE ADDUCT WITH ABTS'''
| |
| | |
| | |
| ==Overview==
| |
| The CotA laccase from the endospore coat of Bacillus subtilis has been crystallized in the presence of the non-catalytic co-oxidant 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and the structure was determined using synchrotron radiation. The binding site for this adduct is well defined and indicates how ABTS, in conjunction with laccases, could act as an oxidative mediator toward non-phenolic moieties. In addition, a dioxygen moiety is clearly defined within the solvent channel oriented toward one of the T3 copper atoms in the trinuclear center.
| |
| | |
| ==About this Structure==
| |
| 1UVW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UVW OCA].
| |
| | |
| ==Reference==
| |
| Substrate and dioxygen binding to the endospore coat laccase from Bacillus subtilis., Enguita FJ, Marcal D, Martins LO, Grenha R, Henriques AO, Lindley PF, Carrondo MA, J Biol Chem. 2004 May 28;279(22):23472-6. Epub 2004 Feb 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14764581 14764581]
| |
| [[Category: Bacillus subtilis]]
| |
| [[Category: Single protein]]
| |
| [[Category: Carrondo, M A.]]
| |
| [[Category: Enguita, F J.]]
| |
| [[Category: Grenha, R.]]
| |
| [[Category: Henriques, A O.]]
| |
| [[Category: Marcal, D.]]
| |
| [[Category: Martins, L O.]]
| |
| [[Category: CU1]]
| |
| [[Category: EBS]]
| |
| [[Category: GOL]]
| |
| [[Category: OXY]]
| |
| [[Category: bacillus subtili]]
| |
| [[Category: laccase]]
| |
| [[Category: multicopper oxidase]]
| |
| [[Category: sporulation]]
| |
| | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:37:28 2008''
| |
This PDB entry is obsolete and replaced by 3zdw