Colicin D: Difference between revisions
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==Synthesis and release== | ==Synthesis and release== | ||
The operon for colicin D is encoded on a plasmid in the cytoplasm of the E. coli. This operon also encodes its [[Colicin Immunity Protein]], [[ImmD]], to protect | The operon for colicin D is encoded on a plasmid in the cytoplasm of the E. coli. This operon also encodes its [[Colicin Immunity Protein]], [[ImmD]], to protect the cell from the tRNase activities of the colicin, alongside a protein to aid the release of the colicin outside the cell. | ||
Once produced, the immunity protein binds to the cytotoxic domain of the colicin and inhibits its enzymatic activity. | Once produced, the immunity protein binds to the cytotoxic domain of the colicin and inhibits its enzymatic activity. | ||
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==Killing Activities== | ==Killing Activities== | ||
Colicin D kills its target cell through its cytotoxic domain which carries [[tRNase activity]]; it cleaves the anticodon of select tRNA molecules, preventing them from being paired with the codon when in the ribosome. This halts protein biosynthesis, ultimately leading to the death of the cell. | |||