Colicin 6: Difference between revisions
New page: Colicin S4 is a type of Colicin, a bacteriocin made by "E. Coli" which acts against other nearby "E. Coli" to kill them by forming a pore in the membrane, leading to depolarisation of ... |
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Colicin | Colicin 6 is a type of [[Colicin]], a bacteriocin made by ''E. coli'' which acts against other nearby ''E. coli'' to kill them by forming a pore in the membrane, leading to depolarisation of the membrane which kills the cell. | ||
==Synthesis and release== | ==Synthesis and release== | ||
The operon for colicin 6 is encoded on a plasmid in the cytoplasm of the E. coli. This operon also encodes its [[Colicin Immunity Protein]], to protect the membrane of the cell from the pore forming activities of the colicin, alongside a protein to aid the release of the colicin outside the cell. | |||
Once produced, the immunity protein inserts into the membrane to prevent pore formation. | |||
==Mechanism of uptake== | ==Mechanism of uptake== | ||
The receptor binding domain of the colicin binds to the outer membrane receptor Tsx on the target cell; it is a constitutively expressed protein parasitised by the colicin. The translocation domain then recruits proteins from the [[Ton]] system to translocate the protein across the membrane and into the cytoplasm. | |||
==Killing Activities== | ==Killing Activities== | ||
Colicin 6 has [[Pore Formation]] activities, whereby it inserts into the membrane of the targeted cell from the cytoplasm, and forms a pore. This causes depolarisation of the membrane, which is needed for the cell to synthesise ATP. Once this process is halted, the cell cannot survive and so dies. | |||