Sandbox 167: Difference between revisions

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Andrea Gorrell, James Jones

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 == Structure ==
+Generally, firefly luciferases have some similarities with Acyl-CoA ligases and some peptide synthetases despite having different cellular effects. In fixing the structure of L. cruciata luciferase, the analog of a potent aminoacyl-tRNA synthetases (DLSA) was successfuly utilized to represent a stable oxyluciferin intermediate.<ref name="structure">PMID:16541080 </ref>.
+The DLSA occupied the active site of the luciferase, which is composed of an α-helix (residues 248-260) and four short β-sheets (residues 286-289, 313-316, 339-342 and 351-353. Ile288 has been implicated as an important residue in determining the hydrophobicity of the active site environment, and through orientation of the product oxyluciferin, the bioluminescent colour. <ref name="structure" />.
-The active site for 2D1S lies within a central area of the protein, and is composed of an α-helix (residues 248-260) and four short β-sheets (residues 286-289, 313-316, 339-342 and 351-353. Ile288 has been implicated as an important residue in determining the hydrophobicity of the active site environment, and through orientation of the product oxyluciferin, the bioluminescent colour. <ref name="main" />.
+[[Image:2d1s active site with ILE288.jpg | thumb |none | upright=3.0 | Figure 1: PYMOL image of 2D1S highlighting active site and Ile288, putatively identified in hydrophobic control of bioluminescent colour.]]
-[[Image:2d1s active site with ILE288.jpg | thumb |none | upright=3.0 | Figure 1: Caption for figure 1]]
- Notes about the image
-Second image
-Notes about the image
-== Luciferase Reaction ==
-Typically, luciferases produce light through a high energy complex with a luciferin cofactor, and Mg-ATP. While the reaction appears to be similiar across all luciferases, species-variants in the luciferin and luciferase structure, and the exact chemical reaction exist.
 == Related Links ==
 [http://www.pymol.org/ Pymol molecular viewer]
-[http://www.pdb.org/pdb/explore/explore.do?structureId=1VPR Protein Data Bank file on 1VPR]
+[http://www.pdb.org/pdb/explore/explore.do?structureId=2D1S Protein Data Bank file on 2D1S]
-[http://www.ncbi.nlm.nih.gov/protein/ABO61076.1?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum NCBI protein entry on ''P. lunula'' luciferase]
+[http://www.ncbi.nlm.nih.gov/protein/CAA59282.1 NCBI protein entry on ''Photinus pyralis'' luciferase, the american firefly]
 == References ==
 <references />