View source for 9ep9


==NMR solution structure of lipid transfer protei Sola l7 from tomato seeds==
<StructureSection load='9ep9' size='340' side='right'caption='[[9ep9]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9ep9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9EP9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ep9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ep9 OCA], [https://pdbe.org/9ep9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ep9 RCSB], [https://www.ebi.ac.uk/pdbsum/9ep9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ep9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A3Q7EJP1_SOLLC A0A3Q7EJP1_SOLLC] Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.[RuleBase:RU000628]
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== Publication Abstract from PubMed ==
Due to the benefits of tomato as an antioxidant and vitamin source, allergy to this vegetable food is a clinically concerning problem. Sola l 7, a class I lipid transfer protein found in tomato seeds, has been identified as an allergen linked to severe anaphylaxis. However, the role of lipid binding in Sola l 7-induced allergy remains unclear. Here, the three-dimensional structure of recombinant Sola l 7 (rSola l 7) has been elucidated using nuclear magnetic resonance spectroscopy (NMR). Its interaction with free fatty acids has been deeply studied; fluorescence emission spectroscopy revealed that different long-chain fatty acids interact with the protein, affecting the only tyrosine residue present in Sola l 7. On the contrary, no changes in the overall secondary structure were observed after the analysis of the circular dichroism spectra in the presence of fatty acids. Unsaturated oleic and linoleic fatty acids presented higher affinity and promoted more significant changes than saturated or short-chain fatty acids. (1)H-(15)N HSQC NMR spectra allowed to determine the regions of the protein that were modified when rSola l 7 interacts with the fatty acids, suggesting epitope modification after the interaction. For corroboration, IgG and IgE binding to rSola l 7 were assessed in the presence of free fatty acids, revealing that both IgE and IgG binding were significantly lower than in their absence, suggesting a potential protective role of unsaturated fatty acids in tomato allergy.

Long-chain fatty acids block allergic reaction against lipid transfer protein Sola l 7 from tomato seeds.,Parron-Ballesteros J, Martin-Pedraza L, Gordo RG, Mayorga C, Pastor-Vargas C, Titaux-Delgado GA, Villalba M, Batanero E, Pantoja-Uceda D, Turnay J Protein Sci. 2024 Sep;33(9):e5154. doi: 10.1002/pro.5154. PMID:39180496<ref>PMID:39180496</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9ep9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Solanum lycopersicum]]
[[Category: Batanero E]]
[[Category: GGordo R]]
[[Category: Mantin-Pedraz L]]
[[Category: Mayorga C]]
[[Category: Pantoja-Uceda D]]
[[Category: Parron-Ballesteros J]]
[[Category: Turnay J]]
[[Category: Villaba M]]