View source for 8t6t


==SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 13 bp R-loop==
<StructureSection load='8t6t' size='340' side='right'caption='[[8t6t]], [[Resolution|resolution]] 3.04&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8t6t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_Lambda Escherichia phage Lambda] and [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8T6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8T6T FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.04&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8t6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8t6t OCA], [https://pdbe.org/8t6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8t6t RCSB], [https://www.ebi.ac.uk/pdbsum/8t6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8t6t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia phage Lambda]]
[[Category: Large Structures]]
[[Category: Streptococcus pyogenes]]
[[Category: Bravo JPK]]
[[Category: Hibshman GN]]
[[Category: Taylor DW]]