View source for 8grf


==Crystal structure of F-box protein in the ternary complex with adaptor protein Skp1(DL) and its substrate==
<StructureSection load='8grf' size='340' side='right'caption='[[8grf]], [[Resolution|resolution]] 2.53&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8grf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GRF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.53&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8grf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8grf OCA], [https://pdbe.org/8grf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8grf RCSB], [https://www.ebi.ac.uk/pdbsum/8grf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8grf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CISY2_YEAST CISY2_YEAST] Peroxisomal citrate synthase involved in the citrate homeostasis (PubMed:25982115, PubMed:3023912). Catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate (PubMed:25982115, PubMed:3023912). Citrate synthase is the rate-limiting enzyme of the tricarboxylic acid (TCA) cycle (Probable).<ref>PMID:25982115</ref> <ref>PMID:3023912</ref> <ref>PMID:3023912</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Deficiencies in mitochondrial protein import are associated with a number of diseases. However, although nonimported mitochondrial proteins are at great risk of aggregation, it remains largely unclear how their accumulation causes cell dysfunction. Here, we show that nonimported citrate synthase is targeted for proteasomal degradation by the ubiquitin ligase SCF(Ucc1). Unexpectedly, our structural and genetic analyses revealed that nonimported citrate synthase appears to form an enzymatically active conformation in the cytosol. Its excess accumulation caused ectopic citrate synthesis, which, in turn, led to an imbalance in carbon flux of sugar, a reduction of the pool of amino acids and nucleotides, and a growth defect. Under these conditions, translation repression is induced and acts as a protective mechanism that mitigates the growth defect. We propose that the consequence of mitochondrial import failure is not limited to proteotoxic insults, but that the accumulation of a nonimported metabolic enzyme elicits ectopic metabolic stress.

Defective import of mitochondrial metabolic enzyme elicits ectopic metabolic stress.,Nishio K, Kawarasaki T, Sugiura Y, Matsumoto S, Konoshima A, Takano Y, Hayashi M, Okumura F, Kamura T, Mizushima T, Nakatsukasa K Sci Adv. 2023 Apr 14;9(15):eadf1956. doi: 10.1126/sciadv.adf1956. Epub 2023 Apr , 14. PMID:37058555<ref>PMID:37058555</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8grf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Kamura T]]
[[Category: Mizushima T]]
[[Category: Nakatsukasa K]]
[[Category: Nishio K]]