View source for 7k5t


==Bst DNA polymerase I time-resolved structure, 25.5 hr post dATP and dCTP addition==
<StructureSection load='7k5t' size='340' side='right'caption='[[7k5t]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7k5t]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K5T FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DCP:2-DEOXYCYTIDINE-5-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k5t OCA], [https://pdbe.org/7k5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k5t RCSB], [https://www.ebi.ac.uk/pdbsum/7k5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k5t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/E1C9K5_GEOSE E1C9K5_GEOSE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The mechanism of DNA synthesis has been inferred from static structures, but the absence of temporal information raises longstanding questions about the order of events in one of life's most central processes. Here we follow the reaction pathway of a replicative DNA polymerase using time-resolved X-ray crystallography to elucidate the order and transition between intermediates. In contrast to the canonical model, the structural changes observed in the time-lapsed images reveal a catalytic cycle in which translocation precedes catalysis. The translocation step appears to follow a push-pull mechanism where the O-O1 loop of the finger subdomain acts as a pawl to facilitate unidirectional movement along the template with conserved tyrosine residues 714 and 719 functioning as tandem gatekeepers of DNA synthesis. The structures capture the precise order of critical events that may be a general feature of enzymatic catalysis among replicative DNA polymerases.

Following replicative DNA synthesis by time-resolved X-ray crystallography.,Chim N, Meza RA, Trinh AM, Yang K, Chaput JC Nat Commun. 2021 May 11;12(1):2641. doi: 10.1038/s41467-021-22937-z. PMID:33976175<ref>PMID:33976175</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7k5t" style="background-color:#fffaf0;"></div>

==See Also==
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geobacillus stearothermophilus]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Chaput JC]]
[[Category: Chim N]]
[[Category: Meza RA]]
[[Category: Trinh AM]]