View source for 7f1e


==Structure of METTL6 bound with SAM==
<StructureSection load='7f1e' size='340' side='right'caption='[[7f1e]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7f1e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F1E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F1E FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.589&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f1e OCA], [https://pdbe.org/7f1e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f1e RCSB], [https://www.ebi.ac.uk/pdbsum/7f1e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f1e ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/METL6_HUMAN METL6_HUMAN] S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Ser), including tRNA(Ser)(UGA) and tRNA(Ser)(GCU) (PubMed:32923617, PubMed:34922197, PubMed:34268557, PubMed:34862464). Interaction with SARS1/SerRS is required for N(3)-methylcytidine methylation (PubMed:34268557).<ref>PMID:32923617</ref> <ref>PMID:34268557</ref> <ref>PMID:34862464</ref> <ref>PMID:34922197</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
RNA modifications play important roles in mediating the biological functions of RNAs. 3-methylcytidine (m3C), albeit less abundant, is found to exist extensively in tRNAs, rRNAs and mRNAs. Human METTL6 is a m(3)C methyltransferase for tRNAs, including tRNA(SER(UGA)). We solved the structure of human METTL6 in the presence of S-adenosyl-L-methionine and found by enzyme assay that recombinant human METTL6 is active towards tRNA(SER(UGA)). Structural analysis indicated the detailed interactions between S-adenosyl-L-methionine and METTL6, and suggested potential tRNA binding region on the surface of METTL6. The structural research, complemented by biochemistry enzyme assay, will definitely shed light on the design of potent inhibitors for METTL6 in near future.

Structural basis for METTL6-mediated m3C RNA methylation.,Li S, Zhou H, Liao S, Wang X, Zhu Z, Zhang J, Xu C Biochem Biophys Res Commun. 2022 Jan 22;589:159-164. doi:, 10.1016/j.bbrc.2021.12.013. Epub 2021 Dec 13. PMID:34922197<ref>PMID:34922197</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7f1e" style="background-color:#fffaf0;"></div>

==See Also==
*[[TRNA methyltransferase 3D structures|TRNA methyltransferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Li S]]
[[Category: Liao S]]
[[Category: Xu C]]