View source for 7drs


==Structure of SspE_40224==
<StructureSection load='7drs' size='340' side='right'caption='[[7drs]], [[Resolution|resolution]] 3.42&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7drs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_yokosukanensis Streptomyces yokosukanensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DRS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DRS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.42&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7drs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7drs OCA], [https://pdbe.org/7drs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7drs RCSB], [https://www.ebi.ac.uk/pdbsum/7drs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7drs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A6I8WFL9_9ACTN A0A6I8WFL9_9ACTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DNA phosphorothioate (PT) modification, with a nonbridging phosphate oxygen substituted by sulfur, represents a widespread epigenetic marker in prokaryotes and provides protection against genetic parasites. In the PT-based defense system Ssp, SspABCD confers a single-stranded PT modification of host DNA in the 5'-C(PS)CA-3' motif and SspE impedes phage propagation. SspE relies on PT modification in host DNA to exert antiphage activity. Here, structural and biochemical analyses reveal that SspE is preferentially recruited to PT sites mediated by the joint action of its N-terminal domain (NTD) hydrophobic cavity and C-terminal domain (CTD) DNA binding region. PT recognition enlarges the GTP-binding pocket, thereby increasing GTP hydrolysis activity, which subsequently triggers a conformational switch of SspE from a closed to an open state. The closed-to-open transition promotes the dissociation of SspE from self PT-DNA and turns on the DNA nicking nuclease activity of CTD, enabling SspE to accomplish self-nonself discrimination and limit phage predation, even when only a small fraction of modifiable consensus sequences is PT-protected in a bacterial genome.

Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE.,Gao H, Gong X, Zhou J, Zhang Y, Duan J, Wei Y, Chen L, Deng Z, Wang J, Chen S, Wu G, Wang L Nat Commun. 2022 Nov 9;13(1):6773. doi: 10.1038/s41467-022-34505-0. PMID:36351933<ref>PMID:36351933</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7drs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces yokosukanensis]]
[[Category: Chen S]]
[[Category: Haiyan G]]
[[Category: Jinchuan Z]]
[[Category: Wang L]]
[[Category: Wu G]]