View source for 7b1b


==Cryo-EM of Aedes Aegypti Toll5A dimer bound to Spz1C==
<StructureSection load='7b1b' size='340' side='right'caption='[[7b1b]], [[Resolution|resolution]] 4.23&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7b1b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aedes_aegypti Aedes aegypti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B1B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.23&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b1b OCA], [https://pdbe.org/7b1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b1b RCSB], [https://www.ebi.ac.uk/pdbsum/7b1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b1b ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Aedes aegypti has evolved to become an efficient vector for arboviruses but the mechanisms of host-pathogen tolerance are unknown. Immunoreceptor Toll and its ligand Spaetzle have undergone duplication which may allow neofunctionalization and adaptation. Here we present cryo-EM structures and biophysical characterisation of low affinity Toll5A complexes that display transient but specific interactions with Spaetzle1C, forming asymmetric complexes, with only one ligand clearly resolved. Loop structures of Spaetzle1C and Toll5A intercalate, temporarily bridging the receptor C-termini to promote signalling. By contrast unbound receptors form head-to-head homodimers that keep the juxtamembrane regions far apart in an inactive conformation. Interestingly the transcriptional signature of Spaetzle1C differs from other Spaetzle cytokines and controls genes involved in innate immunity, metabolism and tissue regeneration. Taken together our results explain how upregulation of Spaetzle1C in the midgut and Toll5A in the salivary gland shape the concomitant immune response.

Structure and dynamics of Toll immunoreceptor activation in the mosquito Aedes aegypti.,Saucereau Y, Wilson TH, Tang MCK, Moncrieffe MC, Hardwick SW, Chirgadze DY, Soares SG, Marcaida MJ, Gay NJ, Gangloff M Nat Commun. 2022 Aug 30;13(1):5110. doi: 10.1038/s41467-022-32690-6. PMID:36042238<ref>PMID:36042238</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7b1b" style="background-color:#fffaf0;"></div>

==See Also==
*[[Toll-like Receptor 3D structures|Toll-like Receptor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aedes aegypti]]
[[Category: Large Structures]]
[[Category: Chirgadze DY]]
[[Category: Gangloff M]]
[[Category: Hardwick SW]]