View source for 6yh0


==Marasmius oreades agglutinin (MOA) in complex with the truncated PVPRAHS synthetic substrate==
<StructureSection load='6yh0' size='340' side='right'caption='[[6yh0]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6yh0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Marasmius_oreades Marasmius oreades] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YH0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CZZ:THIARSAHYDROXY-CYSTEINE'>CZZ</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yh0 OCA], [https://pdbe.org/6yh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yh0 RCSB], [https://www.ebi.ac.uk/pdbsum/6yh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yh0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8X123_9AGAR Q8X123_9AGAR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Marasmius oreades agglutinin (MOA) is the holotype of an emerging family of fungal chimerolectins and an active Ca(2+)/Mn(2+)-dependent protease, which exhibits a unique papain-like fold with special active site features. Here we investigated the functional significance of the structural elements differentiating MOA from other papain-like cysteine proteases. X-ray crystal structures of MOA co-crystallized with two synthetic substrates reveal cleaved peptides bound to the catalytic site, corresponding to the final products of the proteolytic reaction. Anomalous diffraction data on crystals grown in the presence of calcium and manganese, cadmium or zinc resolve the calcium/manganese preference of MOA and elucidate the inhibitory roles of zinc and cadmium towards papain-like cysteine proteases in general. The reported structures, together with activity data of MOA active site variants, point to a conservation of the general proteolysis mechanism established for papain. Ultimately, the findings suggest that papain and the papain-like domain of MOA are the product of convergent evolution.

Crystal structure of MOA in complex with a peptide fragment: A protease caught in flagranti.,Manna D, Cordara G, Krengel U Curr Res Struct Biol. 2020 Apr 22;2:56-67. doi: 10.1016/j.crstbi.2020.04.003., eCollection 2020. PMID:34235469<ref>PMID:34235469</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6yh0" style="background-color:#fffaf0;"></div>

==See Also==
*[[Agglutinin 3D structures|Agglutinin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Marasmius oreades]]
[[Category: Synthetic construct]]
[[Category: Cordara G]]
[[Category: Krengel U]]
[[Category: Manna D]]