View source for 6uxh


==Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP==
<StructureSection load='6uxh' size='340' side='right'caption='[[6uxh]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6uxh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UXH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.858&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uxh OCA], [https://pdbe.org/6uxh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uxh RCSB], [https://www.ebi.ac.uk/pdbsum/6uxh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uxh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0R0IK90_SOYBN A0A0R0IK90_SOYBN] Interconversion of serine and glycine.[RuleBase:RU000585]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Management of the agricultural pathogen soybean cyst nematode (SCN) relies on the use of SCN-resistant soybean cultivars, a strategy that has been failing in recent years. An underutilized source of resistance, in the soybean genotype Peking, is linked to two polymorphisms in serine hydroxy-methyltransferase 8 (SHMT8). SHMT is a pyridoxal 5'-phosphate (PLP)-dependent enzyme that converts L-serine and (6S)-tetrahydrofolate (THF) to glycine and 5,10-methylenetetrahydrofolate. Here, we determined five crystal structures of the 1,884-residue SHMT8 tetramers from the SCN-susceptible cultivar (cv.) Essex and the SCN-resistant cv. Forrest (whose resistance is derived from the SHMT8 polymorphisms in Peking) were determined in complex with various ligands at 1.4 - 2.35 A resolutions. We find that the two Forrest-specific polymorphic substitutions (P130R and N358Y) impact the mobility of a loop near the entrance of the THF-binding site. Ligand-binding and kinetic studies indicate severely reduced affinity for folate and dramatically impaired enzyme activity in Forrest SHMT8. These findings imply widespread effects on folate metabolism in soybean cv. Forrest that have implications for combating the widespread increase in virulent SCN.

Impaired folate binding of serine hydroxymethyltransferase 8 from soybean underlies resistance to the soybean cyst nematode.,Korasick DA, Kandoth PK, Tanner JJ, Mitchum MG, Beamer LJ J Biol Chem. 2020 Feb 2. pii: RA119.012256. doi: 10.1074/jbc.RA119.012256. PMID:32014996<ref>PMID:32014996</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6uxh" style="background-color:#fffaf0;"></div>

==See Also==
*[[Serine hydroxymethyltransferase 3D structures|Serine hydroxymethyltransferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Glycine max]]
[[Category: Large Structures]]
[[Category: Beamer LJ]]
[[Category: Korasick DA]]
[[Category: Tanner JJ]]