View source for 6tqf


==The structure of ABC transporter Rv1819c in AMP-PNP bound state==
<StructureSection load='6tqf' size='340' side='right'caption='[[6tqf]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6tqf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TQF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tqf OCA], [https://pdbe.org/6tqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tqf RCSB], [https://www.ebi.ac.uk/pdbsum/6tqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tqf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BACA_MYCTU BACA_MYCTU] Multi-solute ABC transporter that mediates uptake of unrelated hydrophilic compounds (PubMed:32296172). Can transport vitamin B12 and related corrinoids, and antimicrobial peptides such as bleomycin (PubMed:18996991, PubMed:32296172, PubMed:23407640). Transmembrane domains (TMD) form a pore in the membrane and the ATP-binding domain (NBD) is responsible for energy generation (PubMed:32296172). Contributes to maintenance of chronic infections (PubMed:18996991).<ref>PMID:18996991</ref> <ref>PMID:23407640</ref> <ref>PMID:32296172</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mycobacterium tuberculosis (Mtb) is an obligate human pathogen and the causative agent of tuberculosis(1-3). Although Mtb can synthesize vitamin B12 (cobalamin) de novo, uptake of cobalamin has been linked to pathogenesis of tuberculosis(2). Mtb does not encode any characterized cobalamin transporter(4-6); however, the gene rv1819c was found to be essential for uptake of cobalamin(1). This result is difficult to reconcile with the original annotation of Rv1819c as a protein implicated in the transport of antimicrobial peptides such as bleomycin(7). In addition, uptake of cobalamin seems inconsistent with the amino acid sequence, which suggests that Rv1819c has a bacterial ATP-binding cassette (ABC)-exporter fold(1). Here, we present structures of Rv1819c, which reveal that the protein indeed contains the ABC-exporter fold, as well as a large water-filled cavity of about 7,700 A(3), which enables the protein to transport the unrelated hydrophilic compounds bleomycin and cobalamin. On the basis of these structures, we propose that Rv1819c is a multi-solute transporter for hydrophilic molecules, analogous to the multidrug exporters of the ABC transporter family, which pump out structurally diverse hydrophobic compounds from cells(8-11).

A mycobacterial ABC transporter mediates the uptake of hydrophilic compounds.,Rempel S, Gati C, Nijland M, Thangaratnarajah C, Karyolaimos A, de Gier JW, Guskov A, Slotboom DJ Nature. 2020 Apr;580(7803):409-412. doi: 10.1038/s41586-020-2072-8. Epub 2020 Mar, 25. PMID:32296172<ref>PMID:32296172</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6tqf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Gati C]]
[[Category: Guskov A]]
[[Category: Rempel S]]
[[Category: Slotboom DJ]]