View source for 6t7i


==Structure of yeast 80S ribosome stalled on the CGA-CGA inhibitory codon combination.==
<SX load='6t7i' size='340' side='right' viewer='molstar' caption='[[6t7i]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6t7i]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T7I FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t7i OCA], [https://pdbe.org/6t7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t7i RCSB], [https://www.ebi.ac.uk/pdbsum/6t7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t7i ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS26A_YEAST RS26A_YEAST] 
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== Publication Abstract from PubMed ==
Inhibitory codon pairs and poly(A) tracts within the translated mRNA cause ribosome stalling and reduce protein output. The molecular mechanisms that drive these stalling events, however, are still unknown. Here, we use a combination of in vitro biochemistry, ribosome profiling, and cryo-EM to define molecular mechanisms that lead to these ribosome stalls. First, we use an in vitro reconstituted yeast translation system to demonstrate that inhibitory codon pairs slow elongation rates which are partially rescued by increased tRNA concentration or by an artificial tRNA not dependent on wobble base-pairing. Ribosome profiling data extend these observations by revealing that paused ribosomes with empty A sites are enriched on these sequences. Cryo-EM structures of stalled ribosomes provide a structural explanation for the observed effects by showing decoding-incompatible conformations of mRNA in the A sites of all studied stall- and collision-inducing sequences. Interestingly, in the case of poly(A) tracts, the inhibitory conformation of the mRNA in the A site involves a nucleotide stacking array. Together, these data demonstrate a novel mRNA-induced mechanisms of translational stalling in eukaryotic ribosomes.

Molecular mechanism of translational stalling by inhibitory codon combinations and poly(A) tracts.,Tesina P, Lessen LN, Buschauer R, Cheng J, Wu CC, Berninghausen O, Buskirk AR, Becker T, Beckmann R, Green R EMBO J. 2019 Dec 20:e103365. doi: 10.15252/embj.2019103365. PMID:31858614<ref>PMID:31858614</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6t7i" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[3D sructureseceptor for activated protein kinase C 1|3D sructureseceptor for activated protein kinase C 1]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Becker R]]
[[Category: Beckmann R]]
[[Category: Berninghausen O]]
[[Category: Buschauer R]]
[[Category: Cheng J]]
[[Category: Tesina P]]