View source for 6rjh


==3D structure of horse spleen apoferritin determined using multifunctional graphene supports for electron cryomicroscopy==
<SX load='6rjh' size='340' side='right' viewer='molstar' caption='[[6rjh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6rjh]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RJH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RJH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rjh OCA], [https://pdbe.org/6rjh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rjh RCSB], [https://www.ebi.ac.uk/pdbsum/6rjh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rjh ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/FRIL_HORSE FRIL_HORSE] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
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== Publication Abstract from PubMed ==
With recent technological advances, the atomic resolution structure of any purified biomolecular complex can, in principle, be determined by single-particle electron cryomicroscopy (cryoEM). In practice, the primary barrier to structure determination is the preparation of a frozen specimen suitable for high-resolution imaging. To address this, we present a multifunctional specimen support for cryoEM, comprising large-crystal monolayer graphene suspended across the surface of an ultrastable gold specimen support. Using a low-energy plasma surface modification system, we tune the surface of this support to the specimen by patterning a range of covalent functionalizations across the graphene layer on a single grid. This support design reduces specimen movement during imaging, improves image quality, and allows high-resolution structure determination with a minimum of material and data.

Multifunctional graphene supports for electron cryomicroscopy.,Naydenova K, Peet MJ, Russo CJ Proc Natl Acad Sci U S A. 2019 May 24. pii: 1904766116. doi:, 10.1073/pnas.1904766116. PMID:31127045<ref>PMID:31127045</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6rjh" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ferritin 3D structures|Ferritin 3D structures]]
== References ==
<references/>
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[[Category: Equus caballus]]
[[Category: Large Structures]]
[[Category: Naydenova K]]
[[Category: Peet MJ]]
[[Category: Russo CJ]]