View source for 6i9f


==Solution structure of As-p18 reveals that nematode fatty acid binding proteins exhibit unusual structural features==
<StructureSection load='6i9f' size='340' side='right'caption='[[6i9f]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6i9f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ascaris_suum Ascaris suum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I9F FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i9f OCA], [https://pdbe.org/6i9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i9f RCSB], [https://www.ebi.ac.uk/pdbsum/6i9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i9f ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FABH_ASCSU FABH_ASCSU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Intracellular lipid binding proteins (iLBPs) of the FABP family of animals transport mainly fatty acids or retinoids, are confined to the cytosol, and have highly similar three-dimensional structures. In contrast, nematodes possess fatty acid binding proteins (nemFABPs) that are secreted into the perivitelline fluid surrounding their developing embryos. We report structures of As-p18, a nemFABP of the large intestinal roundworm Ascaris suum, with ligand bound, determined using X-ray crystallography and nuclear magnetic resonance spectroscopy. In common with other FABPs, As-p18 comprises a ten beta-strand barrel capped by two short alpha-helices, with the carboxylate head group of oleate tethered in the interior of the protein. However, As-p18 exhibits two distinctive longer loops between beta-strands not previously seen in a FABP. One of these is adjacent to the presumed ligand entry portal, so may help to target the protein for efficient loading or unloading of ligand. The second, larger loop is at the opposite end of the molecule and has no equivalent in any iLBP structure yet determined. As-p18 preferentially binds a single 18-carbon fatty acid ligand in its central cavity but in an orientation that differs from iLBPs. The unusual structural features of nemFABPs may relate to resourcing of developing embryos of nematodes.

As-p18, an extracellular fatty acid binding protein of nematodes, exhibits unusual structural features.,Ibanez-Shimabukuro M, Rey-Burusco MF, Gabrielsen M, Franchini GR, Riboldi-Tunnicliffe A, Roe AJ, Griffiths K, Cooper A, Corsico B, Kennedy MW, Smith BO Biosci Rep. 2019 Jul 4. pii: BSR20191292. doi: 10.1042/BSR20191292. PMID:31273060<ref>PMID:31273060</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6i9f" style="background-color:#fffaf0;"></div>

==See Also==
*[[Fatty acid-binding protein 3D structures|Fatty acid-binding protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Ascaris suum]]
[[Category: Large Structures]]
[[Category: Corsico B]]
[[Category: Ibanez Shimabukuro M]]
[[Category: Kennedy MW]]
[[Category: Rey Burusco MF]]
[[Category: Smith BO]]