View source for 6gxh


==The hit-and-return system enables efficient time-resolved serial synchrotron crystallography: FAcD 0MS after reaction initiation==
<StructureSection load='6gxh' size='340' side='right' caption='[[6gxh]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6gxh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopa Rhopa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GXH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GXH FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RPA1163 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=258594 RHOPA])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloacetate_dehalogenase Haloacetate dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.3 3.8.1.3] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gxh OCA], [http://pdbe.org/6gxh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gxh RCSB], [http://www.ebi.ac.uk/pdbsum/6gxh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gxh ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/DEHA_RHOPA DEHA_RHOPA]] Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has lower activity towards bromoacetate and chloroacetate.<ref>PMID:21510690</ref> <ref>PMID:21510690</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography.,Schulz EC, Mehrabi P, Muller-Werkmeister HM, Tellkamp F, Jha A, Stuart W, Persch E, De Gasparo R, Diederich F, Pai EF, Miller RJD Nat Methods. 2018 Nov;15(11):901-904. doi: 10.1038/s41592-018-0180-2. Epub 2018, Oct 30. PMID:30377366<ref>PMID:30377366</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6gxh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Haloacetate dehalogenase]]
[[Category: Rhopa]]
[[Category: Diederich, F]]
[[Category: Gasparo, R De]]
[[Category: Mehrabi, P]]
[[Category: Miller, R J.D]]
[[Category: Mueller-Werkmeister, H]]
[[Category: Pai, E F]]
[[Category: Persch, E]]
[[Category: Schulz, E C]]
[[Category: Stuart, W]]
[[Category: Tellkamp, F]]
[[Category: Hydrolase]]
[[Category: Pump-probe experiment]]
[[Category: Structural enzymology]]
[[Category: Time-resolved crystallography]]