View source for 6gsd


==Plantago Major multifunctional oxidoreductase in complex with progesterone and NADP+==
<StructureSection load='6gsd' size='340' side='right' caption='[[6gsd]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6gsd]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GSD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GSD FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=STR:PROGESTERONE'>STR</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5mlm|5mlm]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-beta-hydroxy-Delta(5)-steroid_dehydrogenase 3-beta-hydroxy-Delta(5)-steroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.145 1.1.1.145] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gsd OCA], [http://pdbe.org/6gsd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gsd RCSB], [http://www.ebi.ac.uk/pdbsum/6gsd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gsd ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The short chain dehydrogenase/reductase superfamily (SDR) is a large family of NAD(P)H-dependent enzymes found in all kingdoms of life. SDRs are particularly well-represented in plants, playing diverse roles in both primary and secondary metabolism. In addition, some plant SDRs are also able to catalyse a reductive cyclisation reaction critical for the biosynthesis of the iridoid backbone that contains a fused 5 and 6-membered ring scaffold. Mining the EST database of Plantago major, a medicinal plant that makes iridoids, we identified a putative 5beta-progesterone reductase gene, PmMOR (P. major multisubstrate oxido-reductase), that is 60% identical to the iridoid synthase gene from Catharanthus roseus. The PmMOR protein was recombinantly expressed and its enzymatic activity assayed against three putative substrates, 8-oxogeranial, citral and progesterone. The enzyme demonstrated promiscuous enzymatic activity and was able to not only reduce progesterone and citral, but also to catalyse the reductive cyclisation of 8-oxogeranial. The crystal structures of PmMOR wild type and PmMOR mutants in complex with NADP(+) or NAD(+) and either 8-oxogeranial, citral or progesterone help to reveal the substrate specificity determinants and catalytic machinery of the protein. Site-directed mutagenesis studies were performed and provide a foundation for understanding the promiscuous activity of the enzyme.

A multisubstrate reductase from Plantago major: structure-function in the short chain reductase superfamily.,Fellows R, Russo CM, Silva CS, Lee SG, Jez JM, Chisholm JD, Zubieta C, Nanao MH Sci Rep. 2018 Oct 4;8(1):14796. doi: 10.1038/s41598-018-32967-1. PMID:30287897<ref>PMID:30287897</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6gsd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chisholm, J D]]
[[Category: Fellows, R]]
[[Category: Jez, J M]]
[[Category: Lee, S G]]
[[Category: Nanao, M]]
[[Category: Russo, C M]]
[[Category: Silva, C]]
[[Category: Zubieta, C]]
[[Category: Enzyme evolution]]
[[Category: Iridoid synthase]]
[[Category: Oxidoreductase]]
[[Category: Progesterone reductase]]
[[Category: Sdr]]