View source for 6fjq


==Adenovirus species 48, fiber knob protein==
<StructureSection load='6fjq' size='340' side='right'caption='[[6fjq]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6fjq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_48 Human adenovirus 48]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FJQ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fjq OCA], [https://pdbe.org/6fjq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fjq RCSB], [https://www.ebi.ac.uk/pdbsum/6fjq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fjq ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Adenovirus based vectors are of increasing importance for wide ranging therapeutic applications. As vaccines, vectors derived from human adenovirus species D serotypes 26 and 48 (HAdV-D26/48) are demonstrating promising efficacy as protective platforms against infectious diseases. Significant clinical progress has been made, yet definitive studies underpinning mechanisms of entry, infection, and receptor usage are currently lacking. Here, we perform structural and biological analysis of the receptor binding fiber-knob protein of HAdV-D26/48, reporting crystal structures, and modelling putative interactions with two previously suggested attachment receptors, CD46 and Coxsackie and Adenovirus Receptor (CAR). We provide evidence of a low affinity interaction with CAR, with modelling suggesting affinity is attenuated through extended, semi-flexible loop structures, providing steric hindrance. Conversely, in silico and in vitro experiments are unable to provide evidence of interaction between HAdV-D26/48 fiber-knob with CD46, or with Desmoglein 2. Our findings provide insight into the cell-virus interactions of HAdV-D26/48, with important implications for the design and engineering of optimised Ad-based therapeutics.

Diversity within the adenovirus fiber knob hypervariable loops influences primary receptor interactions.,Baker AT, Greenshields-Watson A, Coughlan L, Davies JA, Uusi-Kerttula H, Cole DK, Rizkallah PJ, Parker AL Nat Commun. 2019 Feb 14;10(1):741. doi: 10.1038/s41467-019-08599-y. PMID:30765704<ref>PMID:30765704</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6fjq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human adenovirus 48]]
[[Category: Large Structures]]
[[Category: Baker, A T]]
[[Category: Parker, A L]]
[[Category: Rizkallah, P J]]
[[Category: Fiber]]
[[Category: Fiber-knob]]
[[Category: High resolution]]
[[Category: Mastadenovirus]]
[[Category: Structure determination. adenovirus]]
[[Category: Tropism determinant]]
[[Category: Viral protein]]