View source for 6eoj


==PolyA polymerase module of the cleavage and polyadenylation factor (CPF) from Saccharomyces cerevisiae==
<SX load='6eoj' size='340' side='right' viewer='molstar' caption='[[6eoj]], [[Resolution|resolution]] 3.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6eoj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EOJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6EOJ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CFT1, YHH1, YDR301W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), YTH1, YPR107C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), PFS2, YNL317W, N0348 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6eoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eoj OCA], [http://pdbe.org/6eoj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eoj RCSB], [http://www.ebi.ac.uk/pdbsum/6eoj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eoj ProSAT]</span></td></tr>
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== Function ==
[[http://www.uniprot.org/uniprot/CFT1_YEAST CFT1_YEAST]] RNA-binding component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Involved in poly(A) site recognition. May be involved in coupling transcription termination and mRNA 3'-end formation.<ref>PMID:12145212</ref> <ref>PMID:8929410</ref>  [[http://www.uniprot.org/uniprot/PFS2_YEAST PFS2_YEAST]] Integral and essential component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. May bridge the CPF and CFIA complexes.<ref>PMID:10619842</ref>  [[http://www.uniprot.org/uniprot/YTH1_YEAST YTH1_YEAST]] RNA-binding component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB.<ref>PMID:12626716</ref>  
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== Publication Abstract from PubMed ==
Newly transcribed eukaryotic pre-mRNAs are processed at their 3'-ends by the ~1 MDa multiprotein cleavage and polyadenylation factor (CPF). CPF cleaves pre-mRNAs, adds a poly(A) tail and triggers transcription termination but it is unclear how its different enzymes are coordinated and assembled. Here, we show that the nuclease, polymerase and phosphatase activities of yeast CPF are organized into three modules. Using cryo-EM, we determine a 3.5 A resolution structure of the ~200 kDa polymerase module. This reveals four beta propellers in an assembly strikingly similar to other protein complexes that bind nucleic acid. Combined with in vitro reconstitution experiments, our data show that the polymerase module brings together factors required for specific and efficient polyadenylation, to help coordinate mRNA 3'-end processing.

Architecture of eukaryotic mRNA 3'-end processing machinery.,Casanal A, Kumar A, Hill CH, Easter AD, Emsley P, Degliesposti G, Gordiyenko Y, Santhanam B, Wolf J, Wiederhold K, Dornan GL, Skehel M, Robinson CV, Passmore LA Science. 2017 Oct 26. pii: eaao6535. doi: 10.1126/science.aao6535. PMID:29074584<ref>PMID:29074584</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6eoj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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[[Category: Baker's yeast]]
[[Category: Large Structures]]
[[Category: Casanal, A]]
[[Category: Emsley, P]]
[[Category: Hill, C H]]
[[Category: Kumar, A]]
[[Category: Passmore, L]]
[[Category: 3'end processing]]
[[Category: Beta-propeller]]
[[Category: Rna binding protein]]
[[Category: Wd40]]
[[Category: Zinc finger]]