View source for 6ac3


==Structure of a natural red emitting luciferase from Phrixothrix hirtus (P3121 crystal form)==
<StructureSection load='6ac3' size='340' side='right'caption='[[6ac3]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ac3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Phrixothrix_hirtus Phrixothrix hirtus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AC3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ac3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ac3 OCA], [https://pdbe.org/6ac3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ac3 RCSB], [https://www.ebi.ac.uk/pdbsum/6ac3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ac3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9U4U7_9COLE Q9U4U7_9COLE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The different colors of light emitted by bioluminescent beetles that use an identical substrate and chemiexcitation reaction sequence to generate light remain a challenging and controversial mechanistic conundrum. The crystal structures of two beetle luciferases with red- and blue-shifted light relative to the green yellow light of the common firefly species provide direct insight into the molecular origin of the bioluminescence color. The structure of a blue-shifted green-emitting luciferase from the firefly Amydetes vivianii is monomeric with a structural fold similar to the previously reported firefly luciferases. The only known naturally red-emitting luciferase from the glow-worm Phrixothrix hirtus exists as tetramers and octamers. Structural and computational analyses reveal varying aperture between the two domains enclosing the active site. Mutagenesis analysis identified two conserved loops that contribute to the color of the emitted light. These results are expected to advance comparative computational studies into the conformational landscape of the luciferase reaction sequence.

Beetle luciferases with naturally red- and blue-shifted emission.,Carrasco-Lopez C, Ferreira JC, Lui NM, Schramm S, Berraud-Pache R, Navizet I, Panjikar S, Naumov P, Rabeh WM Life Sci Alliance. 2018 Aug 16;1(4):e201800072. doi: 10.26508/lsa.201800072., eCollection 2018 Aug. PMID:30456363<ref>PMID:30456363</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ac3" style="background-color:#fffaf0;"></div>

==See Also==
*[[Luciferase 3D structures|Luciferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Phrixothrix hirtus]]
[[Category: Carrasco-Lopez C]]
[[Category: Naumov P]]
[[Category: Panjikar S]]
[[Category: Rabeh W]]